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Transformation of Aspergillus nidulans by using a trpC plasmid.
Open accessAbstract
We constructed a chimeric plasmid carrying a complete copy of the trifunctional trpC gene from the Ascomycete fungus Aspergillus nidulans. This plasmid, designated pHY201, replicates in Escherichia coli, where it confers resistance to ampicillin and chloramphenicol and complements trpC mutants lacking phosphoribosylanthranilate isomerase activity. We used pHY201 to transform an A. nidulans trpC- strain to trpC+ at frequencies of greater than 20 stable transformants per microgram of DNA. Southern blot analysis of DNA from transformants showed that pHY201 DNA had integrated into the A. nidulans chromosomes in a majority of cases. Most of the integration events appeared to occur at the site of the trpC- allele of the recipient strain. In several instances, we succeeded in recovering pHY201, or derivatives thereof, from A. nidulans transformants by restriction endonuclease digestion of chromosomal DNA, ligation, and transformation of E. coli.
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Cited by
Tsai, H.-F., Wheeler, M. H., Chang, Y. C., & Kwon-Chung, K. J. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.20.6469-6477.1999 · Cited by 385
Development of a high-frequency transforming vector for Aspergillus nidulans
Ballance, D. J., & Turner, G. (1985) · Gene · doi:10.1016/0378-1119(85)90187-8 · Cited by 309
Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus
Fuller, K. K., Chen, S., Loros, J. J., & Dunlap, J. C. (2015) · Eukaryotic Cell · doi:10.1128/ec.00107-15 · Cited by 244
Takeshita, N., Higashitsuji, Y., Konzack, S., & Fischer, R. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-06-0523 · Cited by 163
Tsai, H.-F., Fujii, I., Watanabe, A., Wheeler, M. H., Chang, Y. C., Yasuoka, Y., Ebizuka, Y., & Kwon-Chung, K. J. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m101998200 · Cited by 144
Metadata
- Authors
- Yelton, M. M., Hamer, J. E., & Timberlake, W. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-03
- Volume
- 81
- Issue
- 5
- Pages
- 1470-1474
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2071357103
- Cited by
- 777
- References
- 0
- FWCI
- 11.05
- Type
- journal article
- Language
- en
- Open access
- green
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- Yelton, M. M., Hamer, J. E., & Timberlake, W. E. (1984). Transformation of Aspergillus nidulans by using a trpC plasmid. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.5.1470
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