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Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments.
Open accessAbstract
A clone encoding mouse glial fibrillary acidic protein (GFAP) was isolated from a cDNA library constructed so as to express the cloned sequences. The library was screened using a GFAP-specific polyclonal antiserum; a single bacterial colony expressing GFAP was identified. The complete sequence of the cDNA insert in this clone is presented, encompassing 2.5 kilobases and specifying greater than 97% of the GFAP amino acid sequence. The clone includes a long (1.4-kilobase) 3' untranslated region. Within the coding region, the data show extensive homology with other intermediate filament proteins, particularly in those regions predicted to be alpha-helical. RNA blot transfer experiments using the cloned GFAP cDNA probe revealed a single GFAP mRNA species of 2.7 kilobases in mouse brain. Southern blot analysis indicates the existence of at most two genes encoding GFAP in the mouse genome. The mouse GFAP probe cross-hybridizes weakly at high stringency with genomic DNA from diverse eukaryotic species.
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Cited by
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Middeldorp, J., & Hol, E. M. (2011) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2011.01.005 · Cited by 1,080
Eng, L. F. (1985) · Journal of Neuroimmunology · doi:10.1016/s0165-5728(85)80063-1 · Cited by 734
McKeon, R. J., Jurynec, M. J., & Buck, C. R. (1999) · The Journal of Neuroscience · doi:10.1523/jneurosci.19-24-10778.1999 · Cited by 480
Pekny, M., Levéen, P., Pekna, M., Eliasson, C., Berthold, C. H., Westermark, B., & Betsholtz, C. (1995) · The EMBO Journal · doi:10.1002/j.1460-2075.1995.tb07147.x · Cited by 336
Metadata
- Authors
- Lewis, S. A., Balcarek, J. M., Krek, V, Shelanski, M., & Cowan, N. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-05
- Volume
- 81
- Issue
- 9
- Pages
- 2743-2746
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2056328342
- Cited by
- 348
- References
- 0
- FWCI
- 12.27
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments. https://app.cochat.ai/papers/10.1073/pnas.81.9.2743
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- Lewis, S. A., Balcarek, J. M., Krek, V, Shelanski, M., & Cowan, N. J. (1984). Sequence of a cDNA clone encoding mouse glial fibrillary acidic protein: structural conservation of intermediate filaments. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.9.2743
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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