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Cloning and sequence analysis of cDNA for the luminescent protein aequorin.
Open accessAbstract
The luminescent jellyfish Aequorea contains a photoprotein, aequorin, which emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. A cDNA library of Aequorea was constructed and clones carrying the cDNA for the Ca2+-dependent photoprotein were isolated by the method of colony hybridization using synthetic oligonucleotide probes. The primary structure of the protein deduced from the nucleotide sequence showed that the protein is composed of 189 amino acid residues and has three E-F hand structures that are characteristic for Ca2+-binding sites. The sequence also suggested that the protein has hydrophobic regions at which the protein may interact with its functional chromophore, coelenterazine.
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Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Pinton, P., Pozzan, T., & Rizzuto, R. (1998) · The EMBO Journal · doi:10.1093/emboj/17.18.5298 · Cited by 435
Bailes, H. J., & Lucas, R. J. (2013) · Proceedings of the Royal Society B: Biological Sciences · doi:10.1098/rspb.2012.2987 · Cited by 319
Schnitzler, C. E., Pang, K., Powers, M. L., Reitzel, A. M., Ryan, J. F., Simmons, D., Tada, T., Park, M., Gupta, J., Brooks, S. Y., et al. (2012) · BMC Biology · doi:10.1186/1741-7007-10-107 · Cited by 132
The roles of mitochondria in global and local intracellular calcium signalling
Cartes-Saavedra, B., Ghosh, A., & Hajnóczky, G. (2025) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-024-00820-1 · Cited by 126
Inouye, S., Sahara-Miura, Y., Sato, J.-I., & Suzuki, T. (2015) · Protein Expression and Purification · doi:10.1016/j.pep.2015.02.002 · Cited by 49
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Metadata
- Authors
- Inouye, S., Noguchi, M., Sakaki, Y., Takagi, Y., Miyata, T., Iwanaga, S., Miyata, T., & Tsuji, F. I.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-05
- Volume
- 82
- Issue
- 10
- Pages
- 3154-3158
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2081597832
- Cited by
- 327
- References
- 1
- FWCI
- 2.04
- Type
- journal article
- Language
- en
- Open access
- green
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- Inouye, S., Noguchi, M., Sakaki, Y., Takagi, Y., Miyata, T., Iwanaga, S., Miyata, T., & Tsuji, F. I. (1985). Cloning and sequence analysis of cDNA for the luminescent protein aequorin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.10.3154
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