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Rapid transient induction of phenylalanine ammonia-lyase mRNA in elicitor-treated bean cells
Open accessAbstract
DNAs complementary to a size-selected fraction of poly(A) + RNA present in elicitor-treated cells of bean ( Phaseolus vulgaris L.) were inserted into pAT153 and used to transform Escherichia coli strain C600. Five clones were identified by hybrid-selected translation and cross-hybridization that contained sequences complementary to mRNA encoding phenylalanine ammonia-lyase (EC 4.3.1.5), which catalyzes the first reaction of phenylpropanoid biosynthesis. The longest insert contained a single open reading frame of 1520 base pairs together with 223 base pairs of 3′ untranslated sequence. RNA blot hybridization showed that elicitor caused a rapid, marked but transient increase in phenylalanine ammonia-lyase mRNA that was closely correlated with changes in translatable mRNA activity in vitro and enzyme synthesis in vivo . Blot hybridization of newly synthesized mRNA purified by organomercurial affinity chromatography following in vivo pulse-labeling with 4-thiouridine indicates that elicitor caused a rapid stimulation of phenylalanine ammonia-lyase mRNA synthesis as an early in the defense response leading to accumulation of phenylpropanoid-derived phytoalexins.
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Metadata
- Authors
- Edwards, K., Cramer, C. L., Bolwell, G. P., Dixon, R. A., Schuch, W., & Lamb, C. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-10
- Volume
- 82
- Issue
- 20
- Pages
- 6731-6735
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2065301787
- Cited by
- 224
- References
- 0
- FWCI
- 5.40
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Rapid transient induction of phenylalanine ammonia-lyase mRNA in elicitor-treated bean cells. https://app.cochat.ai/papers/10.1073/pnas.82.20.6731
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- Edwards, K., Cramer, C. L., Bolwell, G. P., Dixon, R. A., Schuch, W., & Lamb, C. J. (1985). Rapid transient induction of phenylalanine ammonia-lyase mRNA in elicitor-treated bean cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.20.6731
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