The DOI matches its Crossref record.
Cloning of Ly-5 cDNA.
Open accessAbstract
A notable feature of Ly-5, among immunogenetic systems that identify glycoproteins of the cell surface and define the surface phenotype of cells according to their lineage, is that the Ly-5 locus specifies a range of molecular isoforms that distinguish cells of different stages and branches of hematopoietic development. The composition of the Ly-5 locus is of much interest in regard to how these isoforms are constructed and differentially regulated according to cell lineage. We describe here a cDNA clone, pLy-5-68, that identifies Ly-5. The Ly-5 specificity of the pLy-5-68 clone was first indicated by a restriction fragment length polymorphism (RFLP), which in Southern blotting distinguishes genomic DNA of C57BL/6 (B6) mice (Ly-5a) from that of B6-Ly-5b congeneic mice whose genome is the same as B6 except for the segment of chromosome 1 that bears Ly-5b. For the following reasons it is unlikely that pLy-5-68 represents a gene linked to Ly-5 that was carried over with Ly-5b during serial backcrossing to make the B6-Ly-5b congeneic strain. In all mouse strains tested, the serological Ly-5 allotype (Ly-5.1 vs. Ly-5.2) accorded with the RFLP pattern. Cells of the ST/bJ mouse strain have unique Ly-5 serological reactions and ST/bJ DNA gives a unique (third) RFLP pattern (Ly-5c) with pLy-5-68. All Ly-5+ cell types reacted positively with pLy-5-68 in RNA transfer blotting, and all Ly-5- cell types tested did not. The difference in size of mRNA reactive with pLy-5-68 in cells expressing the 200-kDa Ly-5 isoform as compared with cells expressing the 220-kDa Ly-5 isoform corresponded with the difference in size of the protein components of those isoforms.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Immunosuppressive plasma cells impede T-cell-dependent immunogenic chemotherapy
Shalapour, S., Font-Burgada, J., Di Caro, G., Zhong, Z., Sanchez-Lopez, E., Dhar, D., Willimsky, G., Ammirante, M., Strasner, A., Hansel, D. E., et al. (2015) · Nature · doi:10.1038/nature14395 · Cited by 611
Microbiota-Induced Type I Interferons Instruct a Poised Basal State of Dendritic Cells
Schaupp, L., Muth, S., Rogell, L., Kofoed-Branzk, M., Melchior, F., Lienenklaus, S., Ganal-Vonarburg, S. C., Klein, M., Guendel, F., Hain, T., et al. (2020) · Cell · doi:10.1016/j.cell.2020.04.022 · Cited by 263
Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging
Young, K., Eudy, E., Bell, R., Loberg, M. A., Stearns, T., Sharma, D., Velten, L., Haas, S., Filippi, M.-D., & Trowbridge, J. J. (2021) · Cell Stem Cell · doi:10.1016/j.stem.2021.03.017 · Cited by 216
Expression of Leukocyte Common Antigen (CD45) on Various Human Leukemia/Lymphoma Cell Lines
Nakano, A., Harada, T., Morikawa, S., & Kato, Y. (1990) · Acta Pathologica Japonica · doi:10.1111/j.1440-1827.1990.tb01549.x · Cited by 102
Tuor, M., Stappers, M. H. T., Desgardin, A., Ruchti, F., Sparber, F., Orr, S. J., Gow, N. A. R., & LeibundGut-Landmann, S. (2025) · Mucosal Immunology · doi:10.1016/j.mucimm.2024.11.004 · Cited by 10
Metadata
- Authors
- Shen, F. W., Saga, Y., Litman, G., Freeman, G., Tung, J. S., Cantor, H., & Boyse, E. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-11
- Volume
- 82
- Issue
- 21
- Pages
- 7360-7363
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2083571966
- Cited by
- 160
- References
- 0
- FWCI
- 4.04
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Cloning of Ly-5 cDNA. https://app.cochat.ai/papers/10.1073/pnas.82.21.7360
- The paper
- Shen, F. W., Saga, Y., Litman, G., Freeman, G., Tung, J. S., Cantor, H., & Boyse, E. A. (1985). Cloning of Ly-5 cDNA. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.21.7360
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
