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Identification of calmodulin-dependent protein kinase III and its major Mr 100,000 substrate in mammalian tissues.
Open accessAbstract
A major substrate, Mr 100,00 (100 kDa), for a Ca2+/calmodulin (CaM)-dependent protein kinase found in many mammalian tissues has been purified from rat pancreas. The purified substrate was used to identify and partially purify a CaM-dependent protein kinase (CaM kinase III) from rat pancreas. The physical properties and substrate specificity of CaM kinase III were distinct from those of all known CaM-dependent protein kinases. Only CaM kinase III was able to phosphorylate the 100-kDa protein; synapsin I, phosphorylase b, myosin light chain, and histone were poor substrates for this enzyme. Polyclonal antibodies, raised against the purified 100-kDa protein, recognized the protein in a variety of mammalian tissues and cell lines. Immunoassay revealed that the 100-kDa protein made up 0.3-1.7% of the total cytosolic protein in these samples. Analysis of CaM kinase III revealed that the enzyme had a similar widespread tissue distribution. These results demonstrate the existence of a fifth CaM-dependent protein phosphorylation system present in high levels in animal cells.
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Cites
Bennett, M. K., Erondu, N. E., & Kennedy, M. B. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44239-6 · Cited by 481
Cited by
Hudmon, A., & Schulman, H. (2002) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.71.110601.135410 · Cited by 658
Protein Phosphorylation and Neuronal Function
Browning, M. D., Huganir, R., & Greengard, P. (1985) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1985.tb05468.x · Cited by 526
Phosphorylation of elongation factor 2 by EF-2 kinase affects rate of translation
Ryazanov, A. G., Shestakova, E. A., & Natapov, P. G. (1988) · Nature · doi:10.1038/334170a0 · Cited by 454
Gozuacik, D., & Kimchi, A. (2007) · Current Topics in Developmental Biology · doi:10.1016/s0070-2153(06)78006-1 · Cited by 437
Ca2+/Calmodulin-dependent Protein Kinases
Swulius, M. T., & Waxham, M. N. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-008-8086-2 · Cited by 396
Metadata
- Authors
- Nairn, A. C., Bhagat, B., & Palfrey, H. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-12
- Volume
- 82
- Issue
- 23
- Pages
- 7939-7943
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1992170822
- Cited by
- 218
- References
- 1
- FWCI
- 4.08
- Type
- journal article
- Language
- en
- Open access
- green
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- Nairn, A. C., Bhagat, B., & Palfrey, H. C. (1985). Identification of calmodulin-dependent protein kinase III and its major Mr 100,000 substrate in mammalian tissues. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.23.7939
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
