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TnphoA: a transposon probe for protein export signals.
Open accessAbstract
We constructed a derivative of transposon Tn5 that permits the generation of hybrid proteins composed of alkaline phosphatase (EC 3.1.3.1) lacking its signal peptide fused to amino-terminal sequences of other proteins. Such a hybrid gives alkaline phosphatase activity if the protein fused to alkaline phosphatase contributes sequences that promote export and thus compensate for the missing alkaline phosphatase signal peptide. Fusions to both a secreted periplasmic protein and a complex cytoplasmic membrane protein led to alkaline phosphatase activity. TnphoA fusions should help localize export signals within the structure of a protein, such as a transmembrane protein, as well as identify new chromosomal genes for secreted and transmembrane proteins.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
Mahajan-Miklos, S., Tan, M.-W., Rahme, L. G., & Ausubel, F. M. (1999) · Cell · doi:10.1016/s0092-8674(00)80958-7 · Cited by 819
Coordinate Regulation and Sensory Transduction in the Control of Bacterial Virulence
Miller, J. F., Mekalanos, J. J., & Falkow, S. (1989) · Science · doi:10.1126/science.2537530 · Cited by 616
Link, A. J., Robison, K., & Church, G. M. (1997) · ELECTROPHORESIS · doi:10.1002/elps.1150180807 · Cited by 342
Nataro, J. P., Deng, Y., Maneval, D. R., German, A. L., Martin, W. C., & Levine, M. M. (1992) · Infection and Immunity · doi:10.1128/iai.60.6.2297-2304.1992 · Cited by 303
Identification of a Salmonella typhimurium invasion locus by selection for hyperinvasive mutants
Lee, C. A., Jones, B. D., & Falkow, S. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.5.1847 · Cited by 296
Metadata
- Authors
- Manoil, C., & Beckwith, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-12
- Volume
- 82
- Issue
- 23
- Pages
- 8129-8133
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2095448507
- Cited by
- 1,013
- References
- 1
- FWCI
- 16.26
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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