The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine.
Open accessAbstract
A synthetic DNA based on the known amino acid sequence of the brain/gut peptide cholecystokinin (CCK) was synthesized. This DNA contained deoxyinosines at ambiguous codon positions and was used as a probe to isolate the CCK gene directly from a human genomic library. Nucleotide sequence analysis of the isolated gene revealed that human preprocholecystokinin consists of 115 amino acid residues, with 11 amino acids in common with the human gastrin precursor, another member of the gastrin-CCK family, and that the coding region is separated by a single, long intron. CCK appears to be encoded by a single-copy gene in the haploid human genome, as revealed by genomic Southern hybridization analysis, suggesting that the same gene is expressed both in gut and brain.
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Cites
Rapid similarity searches of nucleic acid and protein data banks.
Wilbur, W. J., & Lipman, D. J. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.3.726 · Cited by 1,546
Cited by
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Caruthers, M. H. (1985) · Science · doi:10.1126/science.3863253 · Cited by 784
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Strott, C. A. (2002) · Endocrine Reviews · doi:10.1210/er.2001-0040 · Cited by 487
An evaluation of the molecular clock hypothesis using mammalian DNA sequences
Li, W.-H., Tanimura, M., & Sharp, P. M. (1987) · Journal of Molecular Evolution · doi:10.1007/bf02603118 · Cited by 450
Touchot, N., Chardin, P., & Tavitian, A. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.23.8210 · Cited by 408
Base pairing involving deoxyinosine: implications for probe design
Martin, F. H., Castro, M. M., Aboul-ela, F., & Tinoco, I. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.24.8927 · Cited by 342
Metadata
- Authors
- Takahashi, Y., Kato, K., Hayashizaki, Y., Wakabayashi, T., Ohtsuka, E., Matsuki, S., Ikehara, M., & Matsubara, K.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-04
- Volume
- 82
- Issue
- 7
- Pages
- 1931-1935
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1986686615
- Cited by
- 242
- References
- 1
- FWCI
- 10.05
- Type
- journal article
- Language
- en
- Open access
- green
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- Takahashi, Y., Kato, K., Hayashizaki, Y., Wakabayashi, T., Ohtsuka, E., Matsuki, S., Ikehara, M., & Matsubara, K. (1985). Molecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosine. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.7.1931
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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