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Hydroxyl radical "footprinting": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein.
Open accessAbstract
A method has been developed for making "footprints" of proteins bound to DNA. The hydroxyl radical, generated by reduction of hydrogen peroxide by iron(II), is the reagent used to cut the DNA. Hydroxyl radical breaks the backbone of DNA with almost no sequence dependence, so all backbone positions may be monitored for contact with protein. In addition to defining the DNA sequence in contact with the protein, hydroxyl radical footprints embody structural information about the DNA-protein complex. For example, hydroxyl radical footprints of the bacteriophage lambda repressor and Cro protein show directly that these proteins are bound to only one side of the DNA helix. Additional contacts of lambda repressor and Cro protein with DNA, not observed by other chemical footprinting methods, are revealed by hydroxyl radical footprinting.
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Cited by
Gene regulation by steroid hormones
Beato, M. (1989) · Cell · doi:10.1016/0092-8674(89)90237-7 · Cited by 3,383
Protein oxidation and peroxidation
Davies, M. J. (2016) · Biochemical Journal · doi:10.1042/bj20151227 · Cited by 952
O'Halloran, T. V., Frantz, B., Shin, M. K., Ralston, D. M., & Wright, J. G. (1989) · Cell · doi:10.1016/0092-8674(89)90990-2 · Cited by 286
Escolar, L., Pérez-Martı́n, J., & de Lorenzo, V. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.2119 · Cited by 173
Metadata
- Authors
- Tullius, T. D., & Dombroski, B. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-08
- Volume
- 83
- Issue
- 15
- Pages
- 5469-5473
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1975329612
- Cited by
- 512
- References
- 0
- FWCI
- 17.16
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Hydroxyl radical "footprinting": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein. https://app.cochat.ai/papers/10.1073/pnas.83.15.5469
- The paper
- Tullius, T. D., & Dombroski, B. A. (1986). Hydroxyl radical "footprinting": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.15.5469
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
