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Expression of the nuclear gene encoding oxygen-evolving enhancer protein 2 is required for high levels of photosynthetic oxygen evolution in Chlamydomonas reinhardtii
Open accessAbstract
We have cloned a cDNA encoding a 20-kDa polypeptide, oxygen-evolving enhancer protein 2 (OEE2), in Chlamydomonas reinhardtii. This polypeptide has been implicated in photosynthetic oxygen evolution, and it is associated with the photosystem II complex, the site of oxygen evolution in all higher plants and algae. The sequence of OEE2 cDNA, the deduced amino acid sequence of the preprotein, the N-terminal protein sequence of mature OEE2 protein, and the coding regions of the single OEE2 gene are presented. The protein is synthesized with a 57-amino acid N-terminal transit peptide that directs the transport of this polypeptide across three cellular membranes. A nuclear mutant of C. reinhardtii, deficient in oxygen-evolving activity, is shown to be specifically missing OEE2 polypeptides. This mutation, which results in the complete absence of all OEE2 mRNA and protein, does not affect the accumulation of other photosystem II polypeptides or their mRNAs.
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“The oxygen-evolving complex is capable of splitting water into oxygen and hydrogen (Mayfield et al., 1987).”
From Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery (2013) · cites this paper as Mayfield et al., 1987
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Metadata
- Authors
- Mayfield, S. P., Rahire, M., Frank, G., Zuber, H., & Rochaix, J. D.
- Affiliations
- University of Geneva (CH)
- ETH Zurich (CH)
- ORCID
- Mayfield, S P
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1987-02
- Volume
- 84
- Issue
- 3
- Pages
- 749-753
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC304293
- OpenAlex
- W2042039129
- Cited by
- 163
- References
- 14
- Citations by year
- 2025: 4 · 2024: 2 · 2023: 6 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2017: 3 · 2016: 6 · 2015: 5 · 2014: 2 · 2013: 6 · 2012: 5
- FWCI
- 7.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (publishedVersion, other-oa)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- National Institute of General Medical Sciences (GM10246-02)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Metalloenzymes and iron-sulfur proteins
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Keywords
- photosynthetic oxygen evolution, photosystem II, Chlamydomonas reinhardtii, cDNA cloning, transit peptide, nuclear genes, nuclear mutants, gene expression
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cell Nucleus, Chlamydomonas, Cloning, Molecular, DNA, Genes, Mutation, Photosynthesis, Plant Proteins, Algal Proteins
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