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Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin.
Open accessAbstract
The transposon TnphoA was used to generate fusions between phoA, the gene for alkaline phosphatase (PhoA), and genes encoding proteins that are secreted by Vibrio cholerae. One of the PhoA+ mutants isolated showed a dramatic reduction in its ability to colonize the intestines of suckling mice. This mutant no longer produced a 20.5-kDa protein (TcpA) that we show is the major subunit of a V. cholerae pilus. Amino-terminal sequence analysis of the TcpA pilus subunit showed that it shares amino acid homology with the pilins produced by several other pathogenic bacteria. The TcpA pilus was coordinately expressed with cholera toxin under various culture conditions, and this effect appeared to be dependent on the transcriptional activator encoded by the toxR gene. We conclude that the toxR gene plays a central role in the transcriptional regulation of multiple virulence genes of V. cholerae.
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Cited by
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Donaldson, G. P., Lee, S. M., & Mazmanian, S. K. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro3552 · Cited by 2,535
Coordinate Regulation and Sensory Transduction in the Control of Bacterial Virulence
Miller, J. F., Mekalanos, J. J., & Falkow, S. (1989) · Science · doi:10.1126/science.2537530 · Cited by 616
Self-citationMembers of the human gut microbiota involved in recovery from Vibrio cholerae infection
Hsiao, A., Ahmed, A. M. S., Subramanian, S., Griffin, N. W., Drewry, L. L., Petri, W. A., Haque, R., Ahmed, T., & Gordon, J. I. (2014) · Nature · doi:10.1038/nature13738 · Cited by 387
Genotypes Associated with Virulence in Environmental Isolates of Vibrio cholerae
Rivera, I. N. G., Chun, J., Huq, A., Sack, R. B., & Colwell, R. R. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.6.2421-2429.2001 · Cited by 357
Thelin, K. H., & Taylor, R. K. (1996) · Infection and Immunity · doi:10.1128/iai.64.7.2853-2856.1996 · Cited by 320
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Metadata
- Authors
- Taylor, R. K., Miller, V. L., Furlong, D. B., & Mekalanos, J. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-05
- Volume
- 84
- Issue
- 9
- Pages
- 2833-2837
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1991373417
- Cited by
- 1,054
- References
- 0
- FWCI
- 19.92
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Taylor, R. K., Miller, V. L., Furlong, D. B., & Mekalanos, J. J. (1987). Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.84.9.2833
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