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Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection
Open accessAbstract
Culture conditions were developed that induce Arabidopsis thaliana (L.) Heynh. root cuttings to regenerate shoots rapidly and at 100% efficiency. The shoots produce viable seeds in vitro or after rooting in soil. A transformation procedure for Arabidopsis root explants based on kanamycin selection was established. By using this regeneration procedure and an Agrobacterium tumor-inducing Ti plasmid carrying a chimeric neomycin phosphotransferase II gene ( neo ), transformed seed-producing plants were obtained with an efficiency between 20% and 80% within 3 months after gene transfer. F 1 seedlings of these transformants showed Mendelian segregation of the kanamycin-resistance trait. The transformation method could be applied to three different Arabidopsis ecotypes. In addition to the neo gene, a chimeric bar gene conferring resistance to the herbicide Basta was introduced into Arabidopsis . The expression of the bar gene was shown by enzymatic assay.
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Citations
Cited by
Floral dip: a simplified method forAgrobacterium‐mediated transformation ofArabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 21,934
Role of arabidopsis MYC and MYB homologs in drought- and abscisic acid-regulated gene expression.
Abe, H., Yamaguchi-Shinozaki, K., Urao, T., Iwasaki, T., Hosokawa, D., & Shinozaki, K. (1997) · The Plant Cell · doi:10.1105/tpc.9.10.1859 · Cited by 1,049
Estévez, J. M., Cantero, A., Reindl, A., Reichler, S., & León, P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m100854200 · Cited by 668
Barton, M. K., & Poethig, R. S. (1993) · Development · doi:10.1242/dev.119.3.823 · Cited by 626
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Yoshida, R., Hobo, T., Ichimura, K., Mizoguchi, T., Takahashi, F., Aronso, J., Ecker, J. R., & Shinozaki, K. (2002) · Plant and Cell Physiology · doi:10.1093/pcp/pcf188 · Cited by 608
Metadata
- Authors
- Valvekens, D., Montagu, M. V., & Lijsebettens, M. V.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-08
- Volume
- 85
- Issue
- 15
- Pages
- 5536-5540
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1975327433
- Cited by
- 1,277
- References
- 0
- FWCI
- 9.28
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Valvekens, D., Montagu, M. V., & Lijsebettens, M. V. (1988). Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.85.15.5536
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
