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Galactose at the nonreducing terminus of O-linked oligosaccharides of mouse egg zona pellucida glycoprotein ZP3 is essential for the glycoprotein's sperm receptor activity.
Open accessAbstract
During fertilization in mice, zona pellucida glycoprotein ZP3 mediates initial sperm-egg interactions by serving as receptor for sperm. Purified egg ZP3, as well as ZP3-derived O-linked oligosaccharides, exhibit sperm receptor activity in vitro. We report that treatment of purified egg ZP3 and ZP3-derived O-linked oligosaccharides with either alpha-galactosidase or galactose oxidase results in loss of sperm receptor activity. In the latter case, sperm receptor activity can be restored to the oxidized glycoprotein and O-linked oligosaccharides by treatment with sodium borohydride. We conclude that galactose, located in alpha-linkage at the nonreducing terminus of O-linked oligosaccharides, is at least one of the sugar determinants on ZP3 responsible for binding of sperm to the zona pellucida.
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Cited by
Thall, A. D., Malý, P., & Lowe, J. B. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.37.21437 · Cited by 401
Glycobiology of sperm-egg interactions in deuterostomes
Mengerink, K. J., & Vacquier, V. D. (2001) · Glycobiology · doi:10.1093/glycob/11.4.37r · Cited by 103
New insights into the molecular mechanisms of sperm-egg interaction
Nixon, B., Aitken, R. J., & McLaughlin, E. A. (2007) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-6552-x · Cited by 103
Towards the Molecular Basis of Sperm and Egg Interaction during Mammalian Fertilization
Wassarman, P. M., & Litscher, E. S. (2001) · Cells Tissues Organs · doi:10.1159/000016804 · Cited by 69
Self-citationA role for carbohydrate recognition in mammalian sperm-egg binding
Clark, G. F. (2014) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2014.06.051 · Cited by 41
Metadata
- Authors
- Bleil, J. D., & Wassarman, P. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-09
- Volume
- 85
- Issue
- 18
- Pages
- 6778-6782
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2065892941
- Cited by
- 342
- References
- 0
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- green
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