The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Shuttle vectors for the archaebacterium Halobacterium volcanii.
Open accessAbstract
Progress in archaebacterial molecular biology requires tools for genetic analysis. We describe vectors that can be selected and maintained in either Halobacterium volcanii or Escherichia coli. A genetic determinant for resistance to the 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor mevinolin was isolated by "shotgun cloning" into a derivative of the endogenous H. volcanii plasmid pHV2, to form pWL2, which transforms sensitive H. volcanii to mevinolin resistance at high frequency. The resistance determinant, portions of pHV2, and an ampicillin- and tetracycline-resistance-conferring pBR322 derivative, pAT153, were ligated together to form the shuttle vectors pWL101 and pWL102. We describe conditions for the use of these vectors and provide preliminary definition of regions essential for drug resistance and for plasmid replication and maintenance.
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Woese, C. R. (1987) · Microbiological Reviews · doi:10.1128/mr.51.2.221-271.1987 · Cited by 5,042
Cited by
Schleper, C., Kubo, K., & Zillig, W. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.16.7645 · Cited by 269
Archaeal genetics — the third way
Allers, T., & Mevarech, M. (2005) · Nature Reviews Genetics · doi:10.1038/nrg1504 · Cited by 265
Leigh, J. A., Albers, S.-V., Atomi, H., & Allers, T. (2011) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2011.00265.x · Cited by 259
The discovery of CRISPR in archaea and bacteria
Mojica, F. J. M., & Rodriguez‐Valera, F. (2016) · The FEBS Journal · doi:10.1111/febs.13766 · Cited by 207
Metadata
- Authors
- Lam, W. L., & Doolittle, W. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-07
- Volume
- 86
- Issue
- 14
- Pages
- 5478-5482
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2042941251
- Cited by
- 199
- References
- 1
- FWCI
- 6.49
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Shuttle vectors for the archaebacterium Halobacterium volcanii. https://app.cochat.ai/papers/10.1073/pnas.86.14.5478
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- Lam, W. L., & Doolittle, W. F. (1989). Shuttle vectors for the archaebacterium Halobacterium volcanii. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.14.5478
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