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Predicting the orientation of eukaryotic membrane-spanning proteins.
Open accessAbstract
We have developed a rule to predict the orientation of the first internal signal-anchor sequence in eukaryotic transmembrane proteins synthesized on the rough endoplasmic reticulum. The difference in the charges of the 15 residues flanking the first internal signal-anchor determines its orientation, with the more positive portion facing the cytosol. In proteins that span the membrane more than once, the orientation of all subsequent transmembrane segments would be determined by that of the most N-terminal one.
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Cited by
Nakai, K., & Horton, P. (1999) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(98)01336-x · Cited by 2,181
Tusnády, G. E., & Simon, I. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.2107 · Cited by 1,079
Hughes, J., Ward, C. J., Peral, B., Aspinwall, R., Clark, K., San Millán, J. L., Gamble, V., & Harris, P. C. (1995) · Nature Genetics · doi:10.1038/ng0695-151 · Cited by 910
Morl, K., Ma, W., Gething, M.-J., & Sambrook, J. (1993) · Cell · doi:10.1016/0092-8674(93)90521-q · Cited by 818
The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake.
Askwith, C., Eide, D., Van Ho, A., Bernard, P. S., Li, L., Davis-Kaplan, S., Sipe, D. M., & Kaplan, J. (1994) · Cell · doi:10.1016/0092-8674(94)90346-8 · Cited by 670
Metadata
- Authors
- Hartmann, E., Rapoport, T. A., & Lodish, H. F.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-08
- Volume
- 86
- Issue
- 15
- Pages
- 5786-5790
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1985899857
- Cited by
- 584
- References
- 0
- FWCI
- 13.27
- Type
- journal article
- Language
- en
- Open access
- green
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- Hartmann, E., Rapoport, T. A., & Lodish, H. F. (1989). Predicting the orientation of eukaryotic membrane-spanning proteins. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.15.5786
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
