Charybdotoxin inhibits proliferation and interleukin 2 production in human peripheral blood lymphocytes.
Open accessAbstract
We demonstrate that blockade of the lymphocyte voltage-gated K+ channel by charybdotoxin (CTX) inhibits lymphocyte mitogenesis. Charybdotoxin blocks conductance with a Ki of 0.3 nM and inhibits mitogen- and antigen-stimulated proliferation with a Ki of 0.5 nM. As opposed to the other blockers of the lymphocyte K+ channel, the inhibition of mitogenesis by CTX can be overcome selectively by exogenous recombinant interleukin 2 (IL-2); endogenous levels of IL-2 in the culture supernatants of stimulated cells are decreased by the presence of CTX. Our results suggest that the voltage-gated K+ channel is either directly or indirectly involved in IL-2 synthesis and/or secretion.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Membrane hyperpolarization at the G1/S checkpoint is generally required for S phase initiation.
“A number of studies suggest that membrane hyperpolarization at the G1/S checkpoint is generally required for S phase initiation.”
From Membrane potential and cancer progression (2013) · cites this paper as Price et al., 1989
Depolarizing the cell membrane halts G1/S progression in glia, Schwann cells, lymphocytes, V79 Chinese hamster lung cells, C1300 mouse neuroblastoma cells, and MCF-7 human breast cancer cells.
“For example, depolarizing the cell membrane halts G1/S progression in glia (Canady et al., 1990), Schwann cells (Wilson and Chiu, 1993), lymphocytes (Price et al., 1989; Freedman et al., 1992; Wang et al., 1992), V79 Chinese hamster lung cells (Sachs et al., 1974), C1300 mouse neuroblastoma cells (Boonstra et al., 1981), and MCF-7 human breast cancer cells (Wonderlin et al., 1995).”
From Membrane potential and cancer progression (2013) · cites this paper as Price et al., 1989
Cited by
Sorted by citations
Membrane potential and cancer progression
Yang, M., & Brackenbury, W. J. (2013) · Frontiers in Physiology · doi:10.3389/fphys.2013.00185 · Cited by 614 · see citing works
Bioelectric controls of cell proliferation: Ion channels, membrane voltage and the cell cycle
Blackiston, D. J., McLaughlin, K. A., & Levin, M. (2009) · Cell Cycle · doi:10.4161/cc.8.21.9888 · Cited by 468 · see citing works
Kv1.3 potassium channels as a therapeutic target in multiple sclerosis
Rangaraju, S., Chi, V., Pennington, M. W., & Chandy, K. G. (2009) · Expert Opinion on Therapeutic Targets · doi:10.1517/14728220903018957 · Cited by 99 · see citing works
Beeton, C., Wulff, H., Singh, S., Botsko, S., Crossley, G., Gutman, G. A., Cahalan, M. D., Pennington, M., & Chandy, K. G. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212868200 · Cited by 90 · see citing works
- Authors
- Price, M., Lee, S. C., & Deutsch, C.
- Affiliations
- University of Pennsylvania (US)
- ORCID
- Deutsch, C
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1989-12
- Volume
- 86
- Issue
- 24
- Pages
- 10171-10175
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC298669
- OpenAlex
- W2052124270
- Cited by
- 183
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 3 · 2014: 3 · 2013: 5 · 2012: 6
- FWCI
- 2.97
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM-41467)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Keywords
- charybdotoxin, voltage-gated potassium channels, lymphocyte proliferation, interleukin-2, mitogenesis, peripheral blood lymphocytes, potassium channel blockers, IL-2 secretion
- MeSH terms
- Cells, Cultured, Humans, Interleukin-2, Kinetics, Lymphocyte Activation, Lymphocytes, Quinine, Scorpion Venoms, T-Lymphocytes, Tetraethylammonium Compounds, 4-Aminopyridine, Charybdotoxin, Tetraethylammonium
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
