CoChat
Sign Up
Cited by 194

The DOI matches its Crossref record.

Distinctive traits of normal and tumor-derived human mammary epithelial cells expressed in a medium that supports long-term growth of both cell types

Band, V, Sager, R·1989·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.86.4.1249
Open this paper in CoChat — free account

Abstract

A medium is described that supports long-term growth in culture of human primary mammary tumor cells, of normal epithelial cells from mammoplasty, and of mammary tumor cell lines. Tumor cells are shown to be distinguishable from normal mammary epithelial cells by morphology, by growth requirements, and by two markers: preferential expression of the HMFG-2 epitope on tumor cells and preferential retention in tumor cell mitochondria of the lipophilic fluorescent dye rhodamine 123. Differential fluorescence of HMFG-2 fluorescein-conjugated antibodies can be used as a basis for separation of normal and tumor cells in a fluorescence-activated cell sorter, as can differential retention of rhodamine 123.

Overview

Integrity signals

Registry

Crossref · 10.1073/pnas.86.4.1249

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • 16A5 is a nontumorigenic, HPV16 E6/E7-immortalized, EGF-dependent human mammary epithelial cell line that displays expected ligand-induced EGFR degradation kinetics.

    “16A5 is a nontumorigenic, human papillomavirus 16 (HPV16) E6/E7-immortalized and EGF-dependent human mammary epithelial cell (MEC) line (55) that displays the expected ligand-induced EGFR degradation kinetics (56–60) (Fig. 1A).”

    From EHD1 and RUSC2 Control Basal Epidermal Growth Factor Receptor Cell Surface Expression and Recycling (2020) · cites this paper as [55]

Citations

Cited by

Sorted by citations

Metadata

Authors
Band, V, & Sager, R.
Affiliations
Division of Cancer Genetics, Dana-Farber Cancer Institute, Boston, MA 02115.
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1989-02
Volume
86
Issue
4
Pages
1249-1253
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.86.4.1249
Cited by
194
References
0
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review