The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Identification and purification of a single-stranded-DNA-specific exonuclease encoded by the recJ gene of Escherichia coli.
Open accessAbstract
The Escherichia coli recJ gene product was overproduced using a plasmid that carries the recJ gene downstream of a strong regulatable promoter and a strong ribosome-binding site. Overexpression of recJ produced a concomitant increase in the levels of single-stranded-DNA-specific nuclease activity present in crude cell extracts. This nuclease activity was purified to homogeneity and found to reside in a 60-kDa polypeptide. This polypeptide was induced with recJ overexpression and had the size and N-terminal amino acid sequence identical to the predicted RecJ protein sequence. The RecJ nuclease degraded linear single-stranded DNA but did not have exonuclease activity on linear double-stranded substrates or endonuclease activity on either single-stranded or double-stranded substrates. The RecJ exonuclease had greater activity on duplex DNA molecules with 5'-rather than 3'-single-stranded tails.
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Biochemistry of homologous recombination in Escherichia coli
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Matsumoto, Y., & Kim, K. (1995) · Science · doi:10.1126/science.7624801 · Cited by 683
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Metadata
- Authors
- Lovett, S. T., & Kolodner, R. D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-04
- Volume
- 86
- Issue
- 8
- Pages
- 2627-2631
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1972974900
- Cited by
- 279
- References
- 15
- FWCI
- 3.22
- Type
- journal article
- Language
- en
- Open access
- green
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- Lovett, S. T., & Kolodner, R. D. (1989). Identification and purification of a single-stranded-DNA-specific exonuclease encoded by the recJ gene of Escherichia coli. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.8.2627
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
