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Cited by 409Advanced Glycation End Products researchChronic Kidney Disease and DiabetesDiabetes Management and Research

The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.

Overexpression of Fibronectin Induced by Diabetes or High Glucose: Phenomenon With a Memory

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Roy, S, Sala, R, Cagliero, E, Lorenzi, M·1990·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.87.1.404PMC PMC53272
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Abstract

To identify events and mechanisms that might contribute to the poor reversibility of diabetic complications, we examined whether diabetes or high glucose induces changes in gene expression and whether such changes outlast the presence of the metabolic abnormalities. The study focused on fibronectin because the increased amounts of this glycoprotein found in diabetic tissues and thickened basement membranes are as yet unexplained. In streptozotocin-induced diabetic rats, fibronectin mRNA levels were increased to 304 +/- 295% of control (mean +/- SD) in the kidney cortex (P less than 0.02), and to 271 +/- 273% of control in the heart (P less than 0.02), while actin mRNA levels remained unchanged. Elevation of fibronectin mRNA persisted for weeks after restoration of near-normoglycemia. In cultured human endothelial cells, high glucose-induced overexpression of fibronectin and collagen IV remained detectable after replating and multiple cell divisions in the absence of high glucose. Cells shifted to normal-glucose medium after prolonged exposure to high glucose also exhibited a proliferative advantage over cells chronically maintained in normal glucose. Thus, diabetes increases fibronectin expression in tissues that are known targets of the complications, and the effect is not readily reversible. The in vitro studies suggest that hyperglycemia may be responsible for these events through induction of self-perpetuating changes in gene expression.

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Research works printed but not linked: 2

  1. [35]Lee, T. S., Saltsman, K. A., Ohashi, H. & King, G. L. (1989) Proc. Natl. Acad. Sci. USA 86, 5141–5145.
  2. J. (1987) Proc. Natl. Acad. Sci. USA 84, 1911–1915.

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Metadata

Authors
Roy, S., Sala, R., Cagliero, E., & Lorenzi, M.
Affiliations
  • Eye Research Institute of Retina Foundation, Boston, MA 02114.
  • Harvard University (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1990-01
Volume
87
Issue
1
Pages
404-408
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.87.1.404
PMC
PMC53272
OpenAlex
W2043406584
Cited by
409
References
43
Citations by year
2026: 3 · 2025: 7 · 2024: 11 · 2023: 8 · 2022: 11 · 2021: 11 · 2020: 12 · 2019: 13 · 2018: 6 · 2017: 21 · 2016: 12 · 2015: 22 · 2014: 24 · 2013: 18 · 2012: 25
FWCI
7.11
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01hl036861-03)
  • National Heart, Lung, and Blood Institute (HL-36861)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
  • Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Management and Research
Keywords
fibronectin, diabetes mellitus, high glucose, hyperglycemia, diabetes complications, endothelial cells, type IV collagen, renal cortex, heart, basement membrane thickening, streptozotocin-induced diabetes, cell proliferation
MeSH terms
Animals, Cells, Cultured, Diabetes Mellitus, Experimental, Endothelium, Vascular, Female, Fibronectins, Gene Expression Regulation, Glucose, Humans, Kidney Cortex, Male, Myocardium, Pregnancy, Rats, Inbred Strains, Reference Values, RNA, Messenger, Transcription, Genetic, Umbilical Veins, Rats

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