The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding.
Open accessAbstract
We have previously identified a small-cell lung cancer cell line (NCI-H209) that expresses an aberrant, underphosphorylated form of the retinoblastoma protein RB1. Molecular analysis of RB1 mRNA from this cell line revealed a single point mutation within exon 21 that resulted in a nonconservative amino acid substitution (cysteine to phenylalanine) at codon 706. Stable expression of this mutant RB1 cDNA in a human cell line lacking endogenous RB1 demonstrated that this amino acid change was sufficient to inhibit phosphorylation. In addition, this cysteine-to-phenylalanine substitution also resulted in loss of RB1 binding to the simian virus 40 large tumor and adenovirus E1A transforming proteins. These results confirm the importance of exon 21 coding sequences and suggest that the cysteine residue at codon 706 may play a role in achieving a specific protein conformation essential for protein-protein interactions.
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Cites
The human papilloma virus-16 E7-oncoprotein is able to bind to the retinoblastoma gene product
Dyson, N., Howley, P. M., Münger, K., & Harlow, E. (1989) · Science · doi:10.1126/science.2537532 · Cited by 3,017
A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma
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Self-citationChen, P.-L., Scully, P., Shew, J.-Y., Wang, J. Y. J., & Lee, W.-H. (1989) · Cell · doi:10.1016/0092-8674(89)90517-5 · Cited by 952
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Cell cycle dependent regulation of phosphorylation of the human retinoblastoma gene product
Mihara, K., Cao, X.-R., Yen, A., Chandler, S., Driscoll, B., Murphree, A. L., T'Ang, A., & Fung, Y.-K. (1989) · Science · doi:10.1126/science.2588006 · Cited by 449
Cited by
Functional interactions of the retinoblastoma protein with mammalian D-type cyclins
Ewen, M. E., Sluss, H. K., Sherr, C. J., Matsushime, H., Kato, J.-Y., & Livingston, D. M. (1993) · Cell · doi:10.1016/0092-8674(93)90136-e · Cited by 1,010
Rb Interacts with Histone Deacetylase to Repress Transcription
Luo, R. X., Postigo, A. A., & Dean, D. C. (1998) · Cell · doi:10.1016/s0092-8674(00)80940-x · Cited by 960
The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines.
Scheffner, M., Münger, K., Byrne, J. C., & Howley, P. M. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.13.5523 · Cited by 849
Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties
Kaelin, W. G., Krek, W., Sellers, W. R., DeCaprio, J. A., Ajchenbaum, F., Fuchs, C. S., Chittenden, T., Li, Y., Farnham, P. J., Blanar, M. A., et al. (1992) · Cell · doi:10.1016/0092-8674(92)90108-o · Cited by 819
Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins.
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Metadata
- Authors
- Kaye, F. J., Kratzke, R. A., Gerster, J. L., & Horowitz, J. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-09
- Volume
- 87
- Issue
- 17
- Pages
- 6922-6926
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2027416462
- Cited by
- 201
- References
- 6
- FWCI
- 8.24
- Type
- journal article
- Language
- en
- Open access
- green
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- Kaye, F. J., Kratzke, R. A., Gerster, J. L., & Horowitz, J. M. (1990). A single amino acid substitution results in a retinoblastoma protein defective in phosphorylation and oncoprotein binding. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.17.6922
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
