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Cited by 176

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Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin.

Open access
Zhang, D, Wadsworth, P, Hepler, P K·1990·Proceedings of the National Academy of Sciences

Abstract

Carboxyfluorescein-labeled brain tubulin has been microinjected into stamen hair cells of Tradescantia, and its distribution during mitosis and cytokinesis was examined using confocal laser scanning fluorescence microscopy. The results show that brain tubulin incorporates into plant microtubules and is utilized throughout mitosis and cytokinesis. Microtubule structures that incorporate brain tubulin include the preprophase band, the perinuclear sheath at late prophase, the kinetochore fibers during prometaphase, metaphase, and anaphase, the interzone spindle during anaphase, and finally the phragmoplast during late anaphase and telophase. All of these microtubule-containing structures and, notably, their transitions from one to another have been observed in single live cells progressing through mitosis and cytokinesis.

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Metadata

Authors
Zhang, D., Wadsworth, P., & Hepler, P. K.
Venue
Proceedings of the National Academy of Sciences
Published
1990-11
Volume
87
Issue
22
Pages
8820-8824
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2135424505
Cited by
176
References
0
FWCI
4.83
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin. https://app.cochat.ai/papers/10.1073/pnas.87.22.8820
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Zhang, D., Wadsworth, P., & Hepler, P. K. (1990). Microtubule dynamics in living dividing plant cells: confocal imaging of microinjected fluorescent brain tubulin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.22.8820

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.