The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.
Open accessAbstract
A target nucleic acid sequence can be replicated (amplified) exponentially in vitro under isothermal conditions by using three enzymatic activities essential to retroviral replication: reverse transcriptase, RNase H, and a DNA-dependent RNA polymerase. By mimicking the retroviral strategy of RNA replication by means of cDNA intermediates, this reaction accumulates cDNA and RNA copies of the original target. Product accumulation is exponential with respect to time, indicating that newly synthesized cDNAs and RNAs function as templates for a continuous series of transcription and reverse transcription reactions. Ten million-fold amplification occurs after a 1- to 2-hr incubation, with an initial rate of amplification of 10-fold every 2.5 min. This self-sustained sequence replication system is useful for the detection and nucleotide sequence analysis of rare RNAs and DNAs. The analogy to aspects of retroviral replication is discussed.
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Cites
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
A detailed model of reverse transcription and tests of crucial aspects
Gilboa, E., Mitra, S. W., Goff, S., & Baltimore, D. (1979) · Cell · doi:10.1016/0092-8674(79)90357-x · Cited by 612
Degradation of DNA RNA Hybrids by Ribonuclease H and DNA Polymerases of Cellular and Viral Origin
Keller, W., & Crouch, R. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.11.3360 · Cited by 222
Cited by
Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805
Directed Evolution of Nucleic Acid Enzymes
Joyce, G. F. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073717 · Cited by 515
Fox, J. D., Han, S., Samuelson, A., Zhang, Y., Neale, M. L., & Westmoreland, D. (2002) · Journal of Clinical Virology · doi:10.1016/s1386-6532(01)00241-4 · Cited by 84
Müller, U. F. (2006) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-006-6047-1 · Cited by 79
Joyce, G. F. (2009) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.2009.74.004 · Cited by 31
Metadata
- Authors
- Guatelli, J. C., Whitfield, K. M., Kwoh, D. Y., Barringer, K. J., Richman, D. D., & Gingeras, T. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-03
- Volume
- 87
- Issue
- 5
- Pages
- 1874-1878
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2062756489
- Cited by
- 540
- References
- 3
- FWCI
- 8.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Guatelli, J. C., Whitfield, K. M., Kwoh, D. Y., Barringer, K. J., Richman, D. D., & Gingeras, T. R. (1990). Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.5.1874
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