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Yeast artificial chromosome libraries containing large inserts from mouse and human DNA.
Open accessAbstract
Yeast artificial chromosome (YAC) libraries have been difficult to construct with average insert sizes greater than 400 kilobase pairs when DNA is size-fractionated in low-melting-point agarose. By using yeast chromosome in mock cloning experiments, we found that polyamines should be present whenever agarose containing high molecular weight DNA is melted to protect DNA from degradation. By incorporating polyamines during the cloning procedure, we constructed YAC libraries from mouse and human DNA with average insert sizes of 700 and 620 kilobase pairs, respectively. Several genome equivalents of these YAC libraries were replicated onto the surface of many duplicate agar plates using a 40,000 multipin transfer device. High-density filter replicas were screened by hybridization, and 70 mouse YAC clones from 31 loci and 132 human YAC clones from 49 loci were isolated.
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Citations
Cited by
Positional cloning of the mouse obese gene and its human homologue
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., & Friedman, J. M. (1994) · Nature · doi:10.1038/372425a0 · Cited by 13,419
Positional Cloning of the Mouse Circadian Gene
King, D. P., Zhao, Y., Sangoram, A. M., Wilsbacher, L. D., Tanaka, M., Antoch, M. P., Steeves, T. D. L., Vitaterna, M. H., Kornhauser, J. M., Lowrey, P. L., et al. (1997) · Cell · doi:10.1016/s0092-8674(00)80245-7 · Cited by 1,382
Phenotypes of Mouse diabetes and Rat fatty Due to Mutations in the OB (Leptin) Receptor
Chua, S. C., Chung, W. K., Wu-Peng, X. S., Zhang, Y., Liu, S.-M., Tartaglia, L., & Leibel, R. L. (1996) · Science · doi:10.1126/science.271.5251.994 · Cited by 1,075
Bashir, R., Britton, S., Strachan, T., Keers, S., Vafiadaki, E., Lako, M., Richard, I., Marchand, S., Bourg, N., Argov, Z., et al. (1998) · Nature Genetics · doi:10.1038/1689 · Cited by 688
Continuum of overlapping clones spanning the entire human chromosome 21q
Chumakov, I., Rigault, P., Guillou, S., Ougen, P., Billaut, A., Guasconi, G., Gervy, P., LeGall, I., Soularue, P., Grinas, L., et al. (1992) · Nature · doi:10.1038/359380a0 · Cited by 388
Metadata
- Authors
- Larin, Z., Monaco, A. P., & Lehrach, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-05-15
- Volume
- 88
- Issue
- 10
- Pages
- 4123-4127
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2082643661
- Cited by
- 327
- References
- 0
- FWCI
- 14.33
- Type
- journal article
- Language
- en
- Open access
- green
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- Larin, Z., Monaco, A. P., & Lehrach, H. (1991). Yeast artificial chromosome libraries containing large inserts from mouse and human DNA. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.10.4123
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