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Isolation and expression of a cDNA encoding Renilla reniformis luciferase.
Open accessAbstract
Renilla reniformis is an anthozoan coelenterate capable of exhibiting bioluminescence. Bioluminescence in Renilla results from the oxidation of coelenterate luciferin (coelenterazine) by luciferase [Renilla-luciferin:oxygen 2-oxidoreductase (decarboxylating), EC 1.13.12.5]. In vivo, the excited state luciferin-luciferase complex undergoes the process of nonradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, Renilla luciferase emits blue light in the absence of any green fluorescent protein. A Renilla cDNA library has been constructed in lambda gt11 and screened by plaque hybridization with two oligonucleotide probes. We report here the isolation and characterization of a luciferase cDNA and its gene product. The recombinant luciferase expressed in Escherichia coli is identical to native luciferase as determined by SDS/PAGE, immunoblot analysis, and bioluminescence emission characteristics.
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Cited by
Advances in In Vivo Bioluminescence Imaging of Gene Expression
Contag, C. H., & Bachmann, M. H. (2002) · Annual Review of Biomedical Engineering · doi:10.1146/annurev.bioeng.4.111901.093336 · Cited by 870
Yu, Y. A., Shabahang, S., Timiryasova, T. M., Zhang, Q., Beltz, R., Gentschev, I., Goebel, W., & Szalay, A. A. (2004) · Nature Biotechnology · doi:10.1038/nbt937 · Cited by 423
Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output
Loening, A. M. (2006) · Protein Engineering Design and Selection · doi:10.1093/protein/gzl023 · Cited by 417
Applications of bioluminescence in biotechnology and beyond
Syed, A. J., & Anderson, J. C. (2021) · Chemical Society Reviews · doi:10.1039/d0cs01492c · Cited by 341
Paulmurugan, R., & Gambhir, S. S. (2003) · Analytical Chemistry · doi:10.1021/ac020731c · Cited by 310
Metadata
- Authors
- Lorenz, W. W., McCann, R. O., Longiaru, M., & Cormier, M. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-05-15
- Volume
- 88
- Issue
- 10
- Pages
- 4438-4442
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2106685495
- Cited by
- 366
- References
- 0
- FWCI
- 0.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Lorenz, W. W., McCann, R. O., Longiaru, M., & Cormier, M. J. (1991). Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.10.4438
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