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Thermal response of rat fibroblasts stably transfected with the human 70-kDa heat shock protein-encoding gene.
Open accessAbstract
The major heat shock protein hsp70 is synthesized by cells of a wide variety of organisms in response to heat shock or other environmental stresses and is assumed to play an important role in protecting cells from thermal stress. We have tested this hypothesis directly by transfecting a constitutively expressed recombinant human hsp70-encoding gene into rat fibroblasts and examining the relationship between the levels of human hsp70 expressed and thermal resistance of the stably transfected rat cells. Successful transfection and expression of the gene for human hsp70 were characterized by RNA hybridization analysis, two-dimensional gel electrophoresis, and immunoblot analysis. When individual cloned cell lines were exposed to 45 degrees C and their thermal survivals were determined by colony-formation assay, we found that the expression of human hsp70 conferred heat resistance to the rat cells. These results reinforce the hypothesis that hsp70 has a protective function against thermal stress.
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Cited by
Feder, M. E., & Hofmann, G. E. (1999) · Annual Review of Physiology · doi:10.1146/annurev.physiol.61.1.243 · Cited by 4,287
Parsell, D. A., & Lindquist, S. (1993) · Annual Review of Genetics · doi:10.1146/annurev.ge.27.120193.002253 · Cited by 2,273
The heat shock protein 70 family: Highly homologous proteins with overlapping and distinct functions
Daugaard, M., Rohde, M., & Jäättelä, M. (2007) · FEBS Letters · doi:10.1016/j.febslet.2007.05.039 · Cited by 1,148
Role of the Human Heat Shock Protein hsp70 in Protection against Stress-Induced Apoptosis
Mosser, D. D., Caron, A. W., Bourget, L., Denis-Larose, C., & Massie, B. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.9.5317 · Cited by 952
Marber, M. S., Mestril, R., Chi, S. H., Sayen, M. R., Yellon, D. M., & Dillmann, W. H. (1995) · Journal of Clinical Investigation · doi:10.1172/jci117815 · Cited by 843
Metadata
- Authors
- Li, G. C., Li, L. G., Liu, Y. K., Mak, J. Y., Chen, L. L., & Lee, W. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1991-03
- Volume
- 88
- Issue
- 5
- Pages
- 1681-1685
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2003088600
- Cited by
- 357
- References
- 0
- FWCI
- 9.89
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Li, G. C., Li, L. G., Liu, Y. K., Mak, J. Y., Chen, L. L., & Lee, W. M. (1991). Thermal response of rat fibroblasts stably transfected with the human 70-kDa heat shock protein-encoding gene. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.88.5.1681
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