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Circulatory half-life but not interaction with the lutropin/chorionic gonadotropin receptor is modulated by sulfation of bovine lutropin oligosaccharides.
Open accessAbstract
Certain of the glycoprotein hormones, including bovine lutropin (bLH), bear asparagine-linked oligosaccharides terminating with the sequence SO4-4GalNAc beta 1-4GlcNAc beta 1-2Man alpha. To establish the biologic significance of these sulfate-bearing oligosaccharides we have compared properties of native bLH, desulfated bLH, recombinant bLH produced in Chinese hamster ovary cells that bears asparagine-linked oligosaccharides terminating with sialic acid alpha 2- 3Gal beta 1-4GlcNAc beta 1-2Man alpha rather than sulfated oligosaccharides (bLH/CHO), and desialyzed bLH/CHO. Using cultured MA-10 cells, a Leydig cell tumor line expressing the lutropin/chorionic gonadotropin receptor, we have found no differences in binding, cAMP production, or progesterone production between native and desulfated bLH. Sulfation of bLH oligosaccharides does not, therefore, modulate bLH bioactivity at the level of the lutropin/chorionic gonadotropin receptor. Removal of sulfate from bLH oligosaccharides and sialic acid from bLH/CHO oligosaccharides results in rapid clearance from the circulation by the hepatocyte asialoglycoprotein receptor. Thus sulfate, like sialic acid, prevents clearance from the circulation by the asialoglycoprotein receptor. The rapid removal of desulfated bLH from the circulation causes a 4- to 16-fold increase in the amount of bLH required to stimulate ovulation compared with native bLH. Particularly striking were differences in the metabolic clearance rates for native bLH and bLH/CHO, 7.3% per min and 1.7% per min, respectively. These differences were unexpected because bLH and bLH/CHO do not differ significantly in charge or size. The different metabolic clearance rates obtained for bLH and bLH/CHO indicate that the presence of sulfated rather than sialylated oligosaccharides on bLH results in a shorter circulatory half-life, which has a significant impact on in vivo bioactivity.
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Citations
Cited by
Sulfonation and molecular action
Strott, C. A. (2002) · Endocrine Reviews · doi:10.1210/er.2001-0040 · Cited by 487
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Lee, S. J., Evers, S., Roeder, D., Parlow, A. F., Risteli, J., Risteli, L., Lee, Y. C., Feizi, T., Langen, H., & Nussenzweig, M. C. (2002) · Science · doi:10.1126/science.1069540 · Cited by 484
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Solá, R. J., & Griebenow, K. (2010) · BioDrugs · doi:10.2165/11530550-000000000-00000 · Cited by 440
Congenital disorders of glycosylation: genetic model systems lead the way
Aebi, M. (2001) · Trends in Cell Biology · doi:10.1016/s0962-8924(01)01925-0 · Cited by 167
Molecular basis of lutropin recognition by the mannose/GalNAc-4-SO 4 receptor
Roseman, D. S., & Baenziger, J. U. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.170184597 · Cited by 73
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Metadata
- Authors
- Baenziger, J. U., Kumar, S., Brodbeck, R. M., Smith, P. L., & Beranek, M. C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-01
- Volume
- 89
- Issue
- 1
- Pages
- 334-338
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2081230658
- Cited by
- 173
- References
- 0
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Open access
- green
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- Baenziger, J. U., Kumar, S., Brodbeck, R. M., Smith, P. L., & Beranek, M. C. (1992). Circulatory half-life but not interaction with the lutropin/chorionic gonadotropin receptor is modulated by sulfation of bovine lutropin oligosaccharides. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.89.1.334
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