The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Ethidium bromide provides a simple tool for identifying genuine DNA-independent protein associations.
Open accessAbstract
DNA-dependent and DNA-independent associations of DNA-binding proteins are important in transcriptional regulation. The analysis of DNA-independent associations frequently relies on assaying protein interaction in the absence of target DNA sequences. We have found that contaminating DNA in protein preparations can stabilize DNA-dependent associations that may appear DNA-independent. Three cellular proteins of 70, 85, and 110 kDa coimmunoprecipitated with the octamer motif-binding protein Oct-2 because of the presence of contaminating DNA in the cell extracts. In addition, heterodimer formation between Oct-1 (or Oct-2) and Pit-1 during protein-affinity chromatography was stabilized by the contaminating DNA. In both instances, these DNA-dependent protein associations were selectively inhibited by ethidium bromide in the precipitation reaction without any evident effect on DNA-independent protein associations. Thus, ethidium bromide may serve as a simple and general indicator of DNA-dependent and DNA-independent protein associations.
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Cites
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The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
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Cited by
Substrate Specificities and Identification of Putative Substrates of ATM Kinase Family Members
Kim, S.-T., Lim, D.-S., Canman, C. E., & Kastan, M. B. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.53.37538 · Cited by 810
Giese, K., Kingsley, C., Kirshner, J. R., & Grosschedl, R. (1995) · Genes & Development · doi:10.1101/gad.9.8.995 · Cited by 541
van der Vlag, J., & Otte, A. P. (1999) · Nature Genetics · doi:10.1038/70602 · Cited by 527
Mammalian DNA double-strand break repair protein XRCC4 interacts with DNA ligase IV
Critchlow, S. E., Bowater, R. P., & Jackson, S. P. (1997) · Current Biology · doi:10.1016/s0960-9822(06)00258-2 · Cited by 470
Merika, M., & Orkin, S. H. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.5.2437 · Cited by 462
Metadata
- Authors
- Lai, J. S., & Herr, W.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-08
- Volume
- 89
- Issue
- 15
- Pages
- 6958-6962
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2063683246
- Cited by
- 448
- References
- 39
- FWCI
- 7.89
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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