The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Mechanism of hormone-stimulated lipolysis in adipocytes: translocation of hormone-sensitive lipase to the lipid storage droplet.
Open accessAbstract
Hormone-sensitive lipase activity (HSL), which is found in the supernatant of centrifuged homogenates of lipolytically quiet isolated rat adipocytes, was greatly reduced in or absent from the supernatant of lipolytically stimulated cells. The lipase was purified 100- to 250-fold from the supernatant of lipolytically quiet cells to 10-20% purity by a single passage over phenyl-Sepharose resin with high (greater than 70%) activity yields. Western blotting of adipocyte homogenate fractions with polyclonal antiserum raised against HSL showed that the enzyme shifted quantitatively from the supernatant of control cells to the floating "fat cake" of lipolytically stimulated cells. A similar shift to the fat cake was observed when cells were disrupted by hypotonic lysis and centrifugation rather than by homogenization. We propose that upon lipolytic activation of adipocytes and phosphorylation of HSL by cAMP-dependent protein kinase, the critical event is not an increase in catalytic activity (i.e., turnover number) but a translocation of the lipase to its substrate at the surface of the lipid storage droplet.
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Cites
Metabolism of Isolated Fat Cells
Rodbell, M. (1964) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)51687-2 · Cited by 4,427
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Self-citationBelfrage, P., & Vaughan, M. (1969) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)43094-9 · Cited by 759
Hormone-sensitive Lipase and Monoglyceride Lipase Activities in Adipose Tissue
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Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.
Fredrikson, G., Strålfors, P., Nilsson, N. O., & Belfrage, P. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69164-7 · Cited by 341
Hormone-Sensitive Lipase: Sequence, Expression, and Chromosomal Localization to 19 cent-q13.3
Holm, C., Kirchgessner, T. G., Svenson, K. L., Fredrikson, G., Nilsson, S., Miller, C. G., Shively, J. E., Heinzmann, C., Sparkes, R. S., Mohandas, T., et al. (1988) · Science · doi:10.1126/science.3420405 · Cited by 307
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Self-citationGARTON, A. J., CAMPBELL, D. G., CARLING, D., HARDIE, D. G., COLBRAN, R. J., & YEAMAN, S. J. (1989) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1989.tb14548.x · Cited by 271
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Cited by
Brasaemle, D. L. (2007) · Journal of Lipid Research · doi:10.1194/jlr.r700014-jlr200 · Cited by 992
Role of Caveolae and Caveolins in Health and Disease
Cohen, A. W., Hnasko, R., Schubert, W., & Lisanti, M. P. (2004) · Physiological Reviews · doi:10.1152/physrev.00046.2003 · Cited by 893
Tansey, J. T., Sztalryd, C., Gruia-Gray, J., Roush, D. L., Zee, J. V., Gavrilova, O., Reitman, M. L., Deng, C.-X., Li, C., Kimmel, A. R., et al. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.101042998 · Cited by 734
Self-citationKraemer, F. B., & Shen, W.-J. (2002) · Journal of Lipid Research · doi:10.1194/jlr.r200009-jlr200 · Cited by 511
Signalling Mechanisms Regulating Lipolysis
Carmen, G.-Y., & Víctor, S.-M. (2006) · Cellular Signalling · doi:10.1016/j.cellsig.2005.08.009 · Cited by 429
Metadata
- Authors
- Egan, J. J., Greenberg, A. S., Chang, M. K., Wek, S. A., Moos, M. C., & Londos, C.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-09-15
- Volume
- 89
- Issue
- 18
- Pages
- 8537-8541
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2020389453
- Cited by
- 408
- References
- 17
- FWCI
- 3.16
- Type
- journal article
- Language
- en
- Open access
- green
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- Egan, J. J., Greenberg, A. S., Chang, M. K., Wek, S. A., Moos, M. C., & Londos, C. (1992). Mechanism of hormone-stimulated lipolysis in adipocytes: translocation of hormone-sensitive lipase to the lipid storage droplet. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.89.18.8537
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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