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Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.
Open accessAbstract
A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.
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Cited by
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International Human Genome Sequencing Consortium, Whitehead Institute for Biomedical Research, C. F. G. R., Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C., Zody, M. C., Baldwin, J., Devon, K., Dewar, K., et al. (2001) · Nature · doi:10.1038/35057062 · Cited by 24,216
Self-citationA novel MHC class I-like gene is mutated in patients with hereditary haemochromatosis
Feder, J. N., Gnirke, A., Thomas, W., Tsuchihashi, Z., Ruddy, D. A., Basava, A., Dormishian, F., Domingo, R., Ellis, M. C., Fullan, A., et al. (1996) · Nature Genetics · doi:10.1038/ng0896-399 · Cited by 3,664
Abnormal splicing of the leptin receptor in diabetic mice
Lee, G.-H., Proenca, R., Montez, J. M., Carroll, K. M., Darvishzadeh, J. G., Lee, J. I., & Friedman, J. M. (1996) · Nature · doi:10.1038/379632a0 · Cited by 2,327
Positional Cloning of the Mouse Circadian Gene
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Link, A. J., Phillips, D., & Church, G. M. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.20.6228-6237.1997 · Cited by 874
Metadata
- Authors
- Shizuya, H., Birren, B., Kim, U. J., Mancino, V, Slepak, T., Tachiiri, Y., & Simon, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-09-15
- Volume
- 89
- Issue
- 18
- Pages
- 8794-8797
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1996749102
- Cited by
- 1,671
- References
- 0
- FWCI
- 9.93
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. https://app.cochat.ai/papers/10.1073/pnas.89.18.8794
- The paper
- Shizuya, H., Birren, B., Kim, U. J., Mancino, V, Slepak, T., Tachiiri, Y., & Simon, M. (1992). Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.89.18.8794
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