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Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition.
Open accessAbstract
The tropane alkaloid scopolamine is a medicinally important anticholinergic drug present in several solanaceous plants. Hyoscyamine 6 beta-hydroxylase (EC 1.14.11.11) catalyzes the oxidative reactions in the biosynthetic pathway leading from hyoscyamine to scopolamine. We introduced the hydroxylase gene from Hyoscyamus niger under the control of the cauliflower mosaic virus 35S promoter into hyoscyamine-rich Atropa belladonna by the use of an Agrobacterium-mediated transformation system. A transgenic plant that constitutively and strongly expressed the transgene was selected, first by screening for kanamycin resistance and then by immunoscreening leaf samples with an antibody specific for the hydroxylase. In the primary transformant and its selfed progeny that inherited the transgene, the alkaloid contents of the leaf and stem were almost exclusively scopolamine. Such metabolically engineered plants should prove useful as breeding materials for obtaining improved medicinal components.
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Cited by
Production of plant secondary metabolites: a historical perspective
Bourgaud, F., Gravot, A., Milesi, S., & Gontier, E. (2001) · Plant Science · doi:10.1016/s0168-9452(01)00490-3 · Cited by 1,251
Facchini, P. J. (2001) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.52.1.29 · Cited by 622
Pharmacognosy in the New Millennium: Leadfinding and Biotechnology
Verpoorte, R. (2000) · Journal of Pharmacy and Pharmacology · doi:10.1211/0022357001773931 · Cited by 274
Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals
Verpoorte, R., van der Heijden, R., ten Hoopen, H. J. G., & Memelink, J. (1999) · Biotechnology Letters · doi:10.1023/a:1005502632053 · Cited by 269
Mayer, M. J., Narbad, A., Parr, A. J., Parker, M. L., Walton, N. J., Mellon, F. A., & Michael, A. J. (2001) · The Plant Cell · doi:10.1105/tpc.010063 · Cited by 65
Metadata
- Authors
- Yun, D. J., Hashimoto, T., & Yamada, Y.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1992-12-15
- Volume
- 89
- Issue
- 24
- Pages
- 11799-11803
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1986552953
- Cited by
- 309
- References
- 0
- FWCI
- 2.29
- Type
- journal article
- Language
- en
- Open access
- green
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- Yun, D. J., Hashimoto, T., & Yamada, Y. (1992). Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.89.24.11799
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