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DNA interstrand cross-links of trans-diamminedichloroplatinum(II) are preferentially formed between guanine and complementary cytosine residues.
Open accessAbstract
Bases in the opposite strands of DNA cross-linked by clinically ineffective trans-diamminedichloroplatinum(II) (trans-[Pt(NH3)2Cl2]) have been identified by means of three experimental approaches. These include HPLC analysis of enzymatic digests of synthetic oligonucleotide duplexes containing the interstrand cross-link, footprinting experiments on the interstrand cross-linked oligonucleotide duplexes, and termination of the duplex transcription on trans-[Pt(NH3)2Cl2]-treated fragments of plasmid DNA. The results reveal that deoxyguanine and complementary deoxycytosine residues are preferential binding sites of trans-[Pt(NH3)2Cl2] in the interstrand adducts. The interstrand cross-linking reaction was studied by means of gel electrophoresis for the cis and trans isomers. The rate of formation of interstrand cross-links was lower for the trans isomer; however, trans-[Pt(NH3)2Cl2] formed about twice the amount of interstrand cross-links as compared with the cis isomer after 48 hr. The present results are suggested to be relevant to differences in clinical activity of the two platinum(II) isomers.
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Cited by
Repair of DNA interstrand cross-links
Dronkert, M. L. G., & Kanaar, R. (2001) · Mutation Research/DNA Repair · doi:10.1016/s0921-8777(01)00092-1 · Cited by 555
Formation and Repair of Interstrand Cross-Links in DNA
Noll, D. M., Mason, T. M., & Miller, P. S. (2006) · Chemical Reviews · doi:10.1021/cr040478b · Cited by 484
Guo, Z., & Sadler, P. J. (1999) · Advances in Inorganic Chemistry · doi:10.1016/s0898-8838(08)60271-8 · Cited by 354
Lukin, M., & de los Santos, C. (2006) · Chemical Reviews · doi:10.1021/cr0404646 · Cited by 158
Contributions of DNA interstrand cross-links to aging of cells and organisms
Grillari, J., Katinger, H., & Voglauer, R. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm1065 · Cited by 83
Metadata
- Authors
- Brabec, V, & Leng, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-06
- Volume
- 90
- Issue
- 11
- Pages
- 5345-5349
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2031168662
- Cited by
- 240
- References
- 0
- FWCI
- 2.53
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: DNA interstrand cross-links of trans-diamminedichloroplatinum(II) are preferentially formed between guanine and complementary cytosine residues. https://app.cochat.ai/papers/10.1073/pnas.90.11.5345
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- Brabec, V, & Leng, M. (1993). DNA interstrand cross-links of trans-diamminedichloroplatinum(II) are preferentially formed between guanine and complementary cytosine residues. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.11.5345
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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