The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials.
Open accessAbstract
The CCAAT/enhancer-binding protein (C/EBP) alpha is a leucine zipper protein that is preferentially expressed in certain cell types, such as adipocytes and hepatocytes. Here we show that C/EBP alpha mRNA is translated into two major proteins, C/EBP-42 and C/EBP-30, that differ in their content of N-terminal amino acid sequences. These results are best explained by a ribosome-scanning mechanism in which a fraction of ribosomes ignore the first two AUGs and initiate translation at an AUG located 351 nt downstream of the first one. Because C/EBP-30, the translation product initiated at the third AUG, is devoid of the potent transcription-activation domain contained in C/EBP-42, the former protein stimulates transcription from the mouse albumin promoter much less efficiently than the latter. The gene encoding the liver-enriched transcriptional-activator protein LAP (C/EBP-beta) has also been shown to issue two proteins, LAP and the liver-enriched transcriptional-inhibitory protein LIP, with different transcription-activation potentials. The production of multiple proteins from a single mRNA is not only shared between different C/EBP family members but also appears to be conserved in vertebrate evolution.
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Cites
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Cited by
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Biological Role of the CCAAT/Enhancer-binding Protein Family of Transcription Factors
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Upstream Open Reading Frames as Regulators of mRNA Translation
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Metadata
- Authors
- Ossipow, V, Descombes, P., & Schibler, U.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-09
- Volume
- 90
- Issue
- 17
- Pages
- 8219-8223
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1989481014
- Cited by
- 366
- References
- 21
- FWCI
- 5.62
- Type
- journal article
- Language
- en
- Open access
- green
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- Ossipow, V, Descombes, P., & Schibler, U. (1993). CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.17.8219
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
