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Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria.
Open accessAbstract
Regulation of the direction of flagellar rotation is central to the mechanism of bacterial chemotaxis. The transitions between counterclockwise and clockwise rotation are controlled by a "switch complex" composed of three proteins (FliG, FliM, and FliN) and located at the base of the flagellar motor. The mechanism of function of the switch is unknown. Here we demonstrate that the diffusible clockwise-signal molecule, the CheY protein, binds to the switch, that the primary docking site is FliM, that the extent of CheY binding to FliM is dependent upon the phosphorylation level of CheY, and that it is unaffected by the other two switch proteins. This study provides a biochemical demonstration of binding of a signal molecule to the bacterial switch and demonstrates directly that phosphorylation regulates the activity of this molecule.
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Citations
Cited by
PAS Domains: Internal Sensors of Oxygen, Redox Potential, and Light
Taylor, B. L., & Zhulin, I. B. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.2.479-506.1999 · Cited by 1,632
The Rotary Motor of Bacterial Flagella
Berg, H. C. (2003) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.72.121801.161737 · Cited by 1,457
Signal processing in complex chemotaxis pathways
Porter, S. L., Wadhams, G. H., & Armitage, J. P. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2505 · Cited by 465
Sowa, Y., & Berry, R. M. (2008) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583508004691 · Cited by 435
Büttner, D. (2012) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.05017-11 · Cited by 431
Metadata
- Authors
- Welch, M., Oosawa, K., Aizawa, S., & Eisenbach, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-10
- Volume
- 90
- Issue
- 19
- Pages
- 8787-8791
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2049716406
- Cited by
- 423
- References
- 0
- FWCI
- 8.50
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria. https://app.cochat.ai/papers/10.1073/pnas.90.19.8787
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- Welch, M., Oosawa, K., Aizawa, S., & Eisenbach, M. (1993). Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.19.8787
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