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High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
Open accessAbstract
We report here a 100-fold increased frequency of plastid transformation in tobacco by selection for a chimeric aadA gene encoding aminoglycoside 3"-adenylyltransferase, as compared with that obtained with mutant 16S rRNA genes. Expression of aadA confers resistance to spectinomycin and streptomycin. In transforming plasmid pZS197, a chimeric aadA is cloned between rbcL and open reading frame ORF512 plastid gene sequences. Selection was for spectinomycin resistance after biolistic delivery of pZS197 DNA into leaf cells. DNA gel-blot analysis confirmed incorporation of the chimeric aadA gene into the plastid genome by two homologous recombination events via the flanking plastid gene sequences. The chimeric gene became homoplasmic in the recipient cells and is uniformly transmitted to the maternal seed progeny. The ability to transform routinely plastids of land plants opens the way to manipulate the process of photosynthesis and to incorporate novel genes into the plastid genome of crops.
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Citations
Cited by
PLASTID TRANSFORMATION IN HIGHER PLANTS
Maliga, P. (2004) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.55.031903.141633 · Cited by 486
Self-citationFull crop protection from an insect pest by expression of long double-stranded RNAs in plastids
Zhang, J., Khan, S. A., Hasse, C., Ruf, S., Heckel, D. G., & Bock, R. (2015) · Science · doi:10.1126/science.1261680 · Cited by 471
Particle bombardment and the genetic enhancement of crops: myths and realities
Altpeter, F., Baisakh, N., Beachy, R., Bock, R., Capell, T., Christou, P., Daniell, H., Datta, K., Datta, S., Dix, P. J., et al. (2005) · Molecular Breeding · doi:10.1007/s11032-004-8001-y · Cited by 371
Elimination of deleterious mutations in plastid genomes by gene conversion
Khakhlova, O., & Bock, R. (2006) · The Plant Journal · doi:10.1111/j.1365-313x.2006.02673.x · Cited by 369
Metadata
- Authors
- Svab, Z., & Maliga, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-02
- Volume
- 90
- Issue
- 3
- Pages
- 913-917
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2053167380
- Cited by
- 775
- References
- 0
- FWCI
- 14.25
- Type
- journal article
- Language
- en
- Open access
- green
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- Svab, Z., & Maliga, P. (1993). High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.3.913
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