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Decreased expression of Mac-2 (carbohydrate binding protein 35) and loss of its nuclear localization are associated with the neoplastic progression of colon carcinoma.
Open accessAbstract
The Mac-2 lectin (carbohydrate binding protein 35) is a soluble, 32- to 35-kDa phosphoprotein that binds galactose-containing glycoconjugates. We report here that the colonic epithelium is a major site of Mac-2 expression in vivo based on immunohistochemistry of human tissue specimens. In this epithelium, proliferating cells at the base of the crypts do not express Mac-2 but its expression increases with differentiation along the crypt-to-surface axis. Mac-2 expression is concentrated in the nuclei of these differentiated epithelial cells. The progression from normal mucosa to adenoma to carcinoma is associated with significant changes in Mac-2 nuclear localization and expression. In all adenomas (9/9) and carcinomas (13/13) examined, Mac-2 was not present in the nucleus but was localized in the cytoplasm. Sequencing of Mac-2 cDNAs from normal mucosa and carcinoma revealed no specific mutations that could account for this loss of nuclear localization. We also observed a 5- to 10-fold decrease in Mac-2 mRNA levels in cancer compared to normal mucosa as well as a significant reduction in the amount of Mac-2 protein expressed. These observations suggest that Mac-2 exclusion from the nucleus and its decreased expression may be related to the neoplastic progression of colon cancer.
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Cited by
Galectins: versatile modulators of cell adhesion, cell proliferation, and cell death
Perillo, N. L., Marcus, M. E., & Baum, L. G. (1998) · Journal of Molecular Medicine · doi:10.1007/s001090050232 · Cited by 640
Human Galectin-3 Is a Novel Chemoattractant for Monocytes and Macrophages
Sano, H., Hsu, D. K., Yu, L., Apgar, J. R., Kuwabara, I., Yamanaka, T., Hirashima, M., & Liu, F.-T. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.4.2156 · Cited by 521
Mucins and mucin binding proteins in colorectal cancer
Byrd, J. C., & Bresalier, R. S. (2004) · Cancer and Metastasis Reviews · doi:10.1023/a:1025815113599 · Cited by 490
Targeted Disruption of the Galectin-3 Gene Results in Attenuated Peritoneal Inflammatory Responses
Hsu, D. K., Yang, R.-Y., Pan, Z., Yu, L., Salomon, D. R., Fung-Leung, W.-P., & Liu, F.-T. (2000) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)64975-9 · Cited by 428
Vlassara, H., Li, Y. M., Imani, F., Wojciechowicz, D., Yang, Z., Liu, F.-T., & Cerami, A. (1995) · Molecular Medicine · doi:10.1007/bf03401604 · Cited by 421
Metadata
- Authors
- Lotz, M. M., Andrews, C. W., Korzelius, C. A., Lee, E. C., Steele, G. D., Clarke, A., & Mercurio, A. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-04-15
- Volume
- 90
- Issue
- 8
- Pages
- 3466-3470
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2076428688
- Cited by
- 230
- References
- 0
- FWCI
- 3.62
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Decreased expression of Mac-2 (carbohydrate binding protein 35) and loss of its nuclear localization are associated with the neoplastic progression of colon carcinoma. https://app.cochat.ai/papers/10.1073/pnas.90.8.3466
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- Lotz, M. M., Andrews, C. W., Korzelius, C. A., Lee, E. C., Steele, G. D., Clarke, A., & Mercurio, A. M. (1993). Decreased expression of Mac-2 (carbohydrate binding protein 35) and loss of its nuclear localization are associated with the neoplastic progression of colon carcinoma. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.8.3466
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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