The DOI matches its Crossref record.
Lipid modifications of G proteins: alpha subunits are palmitoylated.
Open accessAbstract
A small number of membrane-associated proteins are reversibly and covalently modified with palmitic acid. Palmitoylation of G-protein alpha and beta subunits was assessed by metabolic labeling of subunits expressed in simian COS cells or insect Sf9 cells. The fatty acid was incorporated into all of the alpha subunits examined (alpha s, alpha o, alpha i1, alpha i2, alpha i3, alpha z, and alpha q), including those that are also myristoylated (alpha o, alpha i, and alpha z). Palmitate was released by treatment with base, suggesting attachment to the protein through a thioester or ester bond. Limited tryptic digestion of activated alpha o and alpha s resulted in release of the amino-terminal portions of the proteins and radioactive palmitate. These data are consistent with palmitoylation of the proteins near their amino termini, most likely on Cys-3. Reversible acylation of G-protein alpha subunits may provide an additional mechanism for regulation of signal transduction.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Role of Lipid Modifications in Targeting Proteins to Detergent-resistant Membrane Rafts
Melkonian, K. A., Ostermeyer, A. G., Chen, J. Z., Roth, M. G., & Brown, D. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.6.3910 · Cited by 629
Human RAS Superfamily Proteins and Related GTPases
Colicelli, J. (2004) · Science's STKE · doi:10.1126/stke.2502004re13 · Cited by 582
Weinstein, L. S., Yu, S., Warner, D. R., & Liu, J. (2001) · Endocrine Reviews · doi:10.1210/edrv.22.5.0439 · Cited by 420
Isoforms of Mammalian Adenylyl Cyclase: Multiplicities of Signaling
Sunahara, R. K. (2002) · Molecular Interventions · doi:10.1124/mi.2.3.168 · Cited by 406
Shenoy-Scaria, A. M., Dietzen, D. J., Kwong, J., Link, D. C., & Lublin, D. M. (1994) · The Journal of cell biology · doi:10.1083/jcb.126.2.353 · Cited by 391
Metadata
- Authors
- Linder, M. E., Middleton, P., Hepler, J. R., Taussig, R., Gilman, A. G., & Mumby, S. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1993-04-15
- Volume
- 90
- Issue
- 8
- Pages
- 3675-3679
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1978686382
- Cited by
- 348
- References
- 0
- FWCI
- 11.62
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Lipid modifications of G proteins: alpha subunits are palmitoylated. https://app.cochat.ai/papers/10.1073/pnas.90.8.3675
- The paper
- Linder, M. E., Middleton, P., Hepler, J. R., Taussig, R., Gilman, A. G., & Mumby, S. M. (1993). Lipid modifications of G proteins: alpha subunits are palmitoylated. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.90.8.3675
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
