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DNA-dependent protein kinase (Ku protein-p350 complex) assembles on double-stranded DNA.
Open accessAbstract
The Ku protein is an autoantigen that consists of 70- and 80-kDa polypeptides. It associates with double-stranded DNA at free ends. In the present study, we examined the ability of anti-Ku antibodies to immunoprecipitate various structures from extracts of HeLa cells prepared at different salt concentrations. Under physiological conditions, these antibodies identified a complex containing the Ku protein and the 350-kDa component (p350) of DNA-dependent protein kinase (DNA-PK), which appeared to be closely associated on the DNA strand. In reconstitution experiments with cell extracts and biochemically purified components, the Ku protein-p350 complex formed only in the presence of double-stranded DNA. The reconstituted complex was catalytically active. Together with previous studies, these results indicate that the Ku protein interacts with DNA to create a binding site for p350 as the DNA-PK holoenzyme assembles.
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Citations
Cited by
The DNA-dependent protein kinase
Smith, G. C. M., & Jackson, S. P. (1999) · Genes & Development · doi:10.1101/gad.13.8.916 · Cited by 887
Repair of ionizing radiation-induced DNA double-strand breaks by non-homologous end-joining
Mahaney, B. L., Meek, K., & Lees-Miller, S. P. (2009) · Biochemical Journal · doi:10.1042/bj20080413 · Cited by 717
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Calabretta, B., & Perrotti, D. (2004) · Blood · doi:10.1182/blood-2003-12-4111 · Cited by 592
A means to a DNA end: the many roles of Ku
Downs, J. A., & Jackson, S. P. (2004) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1367 · Cited by 395
PKBα/Akt1 Acts Downstream of DNA-PK in the DNA Double-Strand Break Response and Promotes Survival
Bozulic, L., Surucu, B., Hynx, D., & Hemmings, B. A. (2008) · Molecular Cell · doi:10.1016/j.molcel.2008.02.024 · Cited by 386
Metadata
- Authors
- Suwa, A., Hirakata, M., Takeda, Y., Jesch, S. A., Mimori, T., & Hardin, J. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-07-19
- Volume
- 91
- Issue
- 15
- Pages
- 6904-6908
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2023324317
- Cited by
- 169
- References
- 0
- FWCI
- 4.90
- Type
- journal article
- Language
- en
- Open access
- green
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- Suwa, A., Hirakata, M., Takeda, Y., Jesch, S. A., Mimori, T., & Hardin, J. A. (1994). DNA-dependent protein kinase (Ku protein-p350 complex) assembles on double-stranded DNA. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.15.6904
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