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Cited by 735Cellular Mechanics and InteractionsCell Adhesion Molecules ResearchSkin and Cellular Biology Research

Alpha 1(E)-catenin is an actin-binding and -bundling protein mediating the attachment of F-actin to the membrane adhesion complex.

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Rimm, D L, Koslov, E R, Kebriaei, P, Cianci, C D, Morrow, J S·1995·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.92.19.8813PMC PMC41057
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Abstract

Calcium-dependent homotypic cell-cell adhesion, mediated by molecules such as E-cadherin, guides the establishment of classical epithelial cell polarity and contributes to the control of migration, growth, and differentiation. These actions involve additional proteins, including alpha- and beta-catenin (or plakoglobin) and p120, as well as linkage to the cortical actin cytoskeleton. The molecular basis for these interactions and their hierarchy of interaction remain controversial. We demonstrate a direct interaction between F-actin and alpha (E)-catenin, an activity not shared by either the cytoplasmic domain of E-cadherin or beta-catenin. Sedimentation assays and direct visualization by transmission electron microscopy reveal that alpha 1(E)-catenin binds and bundles F-actin in vitro with micromolar affinity at a catenin/G-actin monomer ratio of approximately 1:7 (mol/mol). Recombinant human beta-catenin can simultaneously bind to the alpha-catenin/actin complex but does not bind actin directly. Recombinant fragments encompassing the amino-terminal 228 residues of alpha 1(E)-catenin or the carboxyl-terminal 447 residues individually bind actin in cosedimentation assays with reduced affinity compared with the full-length protein, and neither fragment bundles actin. Except for similarities to vinculin, neither region contains sequences homologous to established actin-binding proteins. Collectively these data indicate that alpha 1 (E)-catenin is a novel actin-binding and -bundling protein and support a model in which alpha 1(E)-catenin is responsible for organizing and tethering actin filaments at the zones of E-cadherin-mediated cell-cell contact.

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  • The association of E-cadherin with the actin cytoskeleton is mediated by a-catenin linked to the cadherin via b-catenin.

    “The association of E-cadherin with the actin cytoskeleton is mediated by a-catenin that is linked to the cadherin via b-catenin (Rimm et al, 1995).”

    From E‐cadherin is essential for in vivo epidermal barrier function by regulating tight junctions (2005) · cites this paper as Rimm et al, 1995

    The Abstract (p. 8813) reports that α-catenin binds F-actin and that β-catenin can bind the α-catenin–actin complex. It proposes α-catenin as a tether at E-cadherin-mediated contacts, but does not establish the claimed β-catenin link to E-cadherin; it also presents the tethering mechanism as a model rather than a settled fact. (LLM, conf=0.85)

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Cited by

Sorted by citations

Authors
Rimm, D. L., Koslov, E. R., Kebriaei, P., Cianci, C. D., & Morrow, J. S.
Affiliations
Yale University (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1995-09-12
Volume
92
Issue
19
Pages
8813-8817
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.92.19.8813
PMC
PMC41057
OpenAlex
W2143646667
Cited by
735
References
0
Citations by year
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FWCI
16.75
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Crohn's and Colitis Foundation
  • National Institutes of Health (5p01dk038979-05)
  • National Institute of Diabetes and Digestive and Kidney Diseases (P01-DK-38979, R01-DK-43812, R01DK043812)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
Keywords
β-catenin, E-cadherin, F-actin binding, actin bundling, cell-cell adhesion, epithelial polarity, actin cytoskeleton, calcium-dependent adhesion
MeSH terms
Actins, Animals, Cell Compartmentation, Cells, Cultured, Cytoskeletal Proteins, Dogs, Fluorescent Antibody Technique, Intercellular Junctions, Peptide Fragments, Recombinant Proteins, Structure-Activity Relationship, alpha Catenin

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