Detection of exocytosis at individual pancreatic beta cells by amperometry at a chemically modified microelectrode.
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Amperometry at a carbon fiber microelectrode modified with a composite of ruthenium oxide and cyanoruthenate was used to monitor chemical secretions of single pancreatic beta cells from rats and humans. When the insulin secretagogues glucose, tolbutamide, and K+ were applied to the cell, a series of randomly occurring current spikes was observed. The current spikes were shown to be due to the detection of chemical substances secreted from the cell. Chromatography showed that the primary secreted substance detected by the electrode was insulin. The current spikes were strongly dependent on external Ca2+, had an average area that was independent of the stimulation method, and had an area distribution which corresponded to the distribution of vesicle sizes in beta cells. It was concluded that the spikes were due to the detection of concentration pulses of insulin secreted by exocytosis.
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Modified carbon fibers have been used to detect the release of insulin itself.
“and modified carbon fibers have been used to detect the release of insulin itself (294).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [294]
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- E. J. & Viveros, O. H. (1991) Proc. Natl. Acad. Sci. USA 88, 10754–10758.
- [11]Faulkner, L. R. (1984) Chem. Eng. News 27 (2), 28–45.
- [17]St. Claire, R. L., III (1986) Ph.D. dissertation (Univ. of North Carolina, Chapel Hill).
- [21]Hellman, B. & Gylfe, E. (1986) in Calcium and Cell Function, ed. Cheung, W. Y. (Academic, Orlando, FL), Vol. 6, pp. 253–326.
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Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
- Authors
- Huang, L., Shen, H., Atkinson, M. A., & Kennedy, R. T.
- Affiliations
- University of Florida (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1995-10-10
- Volume
- 92
- Issue
- 21
- Pages
- 9608-9612
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC40851
- OpenAlex
- W1984975071
- Cited by
- 107
- References
- 0
- Citations by year
- 2025: 1 · 2024: 3 · 2022: 1 · 2021: 1 · 2020: 3 · 2019: 1 · 2018: 3 · 2017: 4 · 2016: 1 · 2015: 1 · 2014: 2 · 2013: 4 · 2012: 4
- FWCI
- 2.93
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Eli Lilly and Company
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK046960, R0 1DK46960-01)
- Topics
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Keywords
- exocytosis, pancreatic beta cells, insulin secretion, amperometry, carbon fiber microelectrodes, ruthenium oxide, chemical modification, current spikes, calcium dependence, vesicle size distribution, glucose, tolbutamide, potassium stimulation
- MeSH terms
- Insulin Secretion, Animals, Calcium, Cells, Cultured, Chromatography, High Pressure Liquid, Electric Conductivity, Electrophysiology, Exocytosis, Humans, Insulin, Islets of Langerhans, Microelectrodes, Species Specificity, Rats
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