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Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix.
Open accessAbstract
We have developed a technology for analysis and sorting of live cells according to secreted molecules. An artificial affinity matrix, specific for the secreted product of interest, is created on the cell surface, and the cells are allowed to secrete for a defined time period. The secreted molecules bind to the affinity matrix on the secreting cell and are subsequently labeled with specific fluorescent or magnetic staining reagents for cytometric analysis and cell sorting. Crossfeeding of the secreted products to other cells is prevented by decreasing the permeability of the incubation medium. This approach will have a wide range of applications in biotechnology and biomedical research. Here, we describe analysis and sorting of hybridoma cells, according to secreted antibodies, and of activated T lymphocytes, according to secreted cytokines.
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Cited by
Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., Chang, H., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., et al. (2021) · European Journal of Immunology · doi:10.1002/eji.202170126 · Cited by 531
Self-citationPlasma cell survival is mediated by synergistic effects of cytokines and adhesion-dependent signals
Cassese, G., Arce, S., Hauser, A. E., Lehnert, K., Moewes, B., Mostarac, M., Muehlinghaus, G., Szyska, M., Radbruch, A., & Manz, R. A. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.4.1684 · Cited by 487
Self-citationWolfl, M., Kuball, J., Ho, W. Y., Nguyen, H., Manley, T. J., Bleakley, M., & Greenberg, P. D. (2007) · Blood · doi:10.1182/blood-2006-11-056168 · Cited by 458
T-cell-mediated lysis of endothelial cells in acute coronary syndromes
Nakajima, T., Schulte, S., Warrington, K. J., Kopecky, S. L., Frye, R. L., Goronzy, J. J., & Weyand, C. M. (2002) · Circulation · doi:10.1161/hc0502.103348 · Cited by 356
Advances in Mammalian cell line development technologies for recombinant protein production
Lai, T., Yang, Y., & Ng, S. (2013) · Pharmaceuticals · doi:10.3390/ph6050579 · Cited by 322
Metadata
- Authors
- Manz, R., Assenmacher, M., Pflüger, E., Miltenyi, S., & Radbruch, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1995-03-14
- Volume
- 92
- Issue
- 6
- Pages
- 1921-1925
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2054109196
- Cited by
- 303
- References
- 0
- FWCI
- 6.55
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix. https://app.cochat.ai/papers/10.1073/pnas.92.6.1921
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- Manz, R., Assenmacher, M., Pflüger, E., Miltenyi, S., & Radbruch, A. (1995). Analysis and sorting of live cells according to secreted molecules, relocated to a cell-surface affinity matrix. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.92.6.1921
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
