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DNA analysis and diagnostics on oligonucleotide microchips.
Open accessAbstract
We present a further development in the technology of sequencing by hybridization to oligonucleotide microchips (SHOM) and its application to diagnostics for genetic diseases. A robot has been constructed to manufacture sequencing "microchips." The microchip is an array of oligonucleotides immobilized into gel elements fixed on a glass plate. Hybridization of the microchip with fluorescently labeled DNA was monitored in real time simultaneously for all microchip elements with a two-wavelength fluorescent microscope equipped with a charge-coupled device camera. SHOM has been used to detect beta-thalassemia mutations in patients by hybridizing PCR-amplified DNA with the microchips. A contiguous stacking hybridization technique has been applied for the detection of mutations; it can simplify medical diagnostics and enhance its reliability. The use of multicolor monitoring of contiguous stacking hybridization is suggested for large-scale diagnostics and gene polymorphism studies. Other applications of the SHOM technology are discussed.
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Cited by
Universal DNA microarray method for multiplex detection of low abundance point mutations
Gerry, N. P., Witowski, N. E., Day, J., Hammer, R. P., Barany, G., & Barany, F. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.3063 · Cited by 346
Mining SNPs From EST Databases
Picoult-Newberg, L., Ideker, T. E., Pohl, M. G., Taylor, S. L., Donaldson, M. A., Nickerson, D. A., & Boyce-Jacino, M. (1999) · Genome Research · doi:10.1101/gr.9.2.167 · Cited by 331
The Efficiency of Light-Directed Synthesis of DNA Arrays on Glass Substrates
McGall, G. H., Barone, A. D., Diggelmann, M., Fodor, S. P. A., Gentalen, E., & Ngo, N. (1997) · Journal of the American Chemical Society · doi:10.1021/ja964427a · Cited by 282
Metadata
- Authors
- Yershov, G., Barsky, V, Belgovskiy, A., Kirillov, E., Kreindlin, E., Ivanov, I., Parinov, S., Guschin, D., Drobishev, A., Dubiley, S., & Mirzabekov, A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-05-14
- Volume
- 93
- Issue
- 10
- Pages
- 4913-4918
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1994098923
- Cited by
- 385
- References
- 0
- FWCI
- 10.02
- Type
- journal article
- Language
- en
- Open access
- green
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- Yershov, G., Barsky, V, Belgovskiy, A., Kirillov, E., Kreindlin, E., Ivanov, I., Parinov, S., Guschin, D., Drobishev, A., Dubiley, S., & Mirzabekov, A. (1996). DNA analysis and diagnostics on oligonucleotide microchips. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.10.4913
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