The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent-labeled protein kinase C beta I.
Open accessAbstract
We have devised a microspectroscopic strategy for assessing the intracellular (re)distribution and the integrity of the primary structure of proteins involved in signal transduction. The purified proteins are fluorescent-labeled in vitro and reintroduced into the living cell. The localization and molecular state of fluorescent-labeled protein kinase C beta I isozyme were assessed by a combination of quantitative confocal laser scanning microscopy, fluorescence lifetime imaging microscopy, and novel determinations of fluorescence resonance energy transfer based on photobleaching digital imaging microscopy. The intensity and fluorescence resonance energy transfer efficiency images demonstrate the rapid nuclear translocation and ensuing fragmentation of protein kinase C beta I in BALB/c3T3 fibroblasts upon phorbol ester stimulation, and suggest distinct, compartmentalized roles for the regulatory and catalytic fragments.
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Cites
Castagna, M., Takai, Y., Kaibuchi, K., Sano, K., Kikkawa, U., & Nishizuka, Y. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34459-4 · Cited by 4,725
Cited by
Imaging Protein-Protein Interactions Using Fluorescence Resonance Energy Transfer Microscopy
Kenworthy, A. K. (2001) · Methods · doi:10.1006/meth.2001.1189 · Cited by 532
Imaging biochemistry inside cells
Wouters, F. S., Verveer, P. J., & Bastiaens, P. I. H. (2001) · Trends in Cell Biology · doi:10.1016/s0962-8924(01)01982-1 · Cited by 395
Self-citationKinoshita, A., Fukumoto, H., Shah, T., Whelan, C. M., Irizarry, M. C., & Hyman, B. T. (2003) · Journal of Cell Science · doi:10.1242/jcs.00643 · Cited by 284
Stepwise androgen receptor dimerization
van Royen, M. E., van Cappellen, W. A., de Vos, C., Houtsmuller, A. B., & Trapman, J. (2012) · Journal of Cell Science · doi:10.1242/jcs.096792 · Cited by 143
LRMP inhibits cAMP potentiation of HCN4 channels by disrupting intramolecular signal transduction
(2024) · doi:10.7554/elife.92411.3
Metadata
- Authors
- Bastiaens, P. I., & Jovin, T. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-08-06
- Volume
- 93
- Issue
- 16
- Pages
- 8407-8412
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1965797967
- Cited by
- 168
- References
- 1
- FWCI
- 3.29
- Type
- journal article
- Language
- en
- Open access
- green
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- Bastiaens, P. I., & Jovin, T. M. (1996). Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent-labeled protein kinase C beta I. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.16.8407
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
