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Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine.
Open accessAbstract
Plasmodium falciparum malaria parasites were transformed with plasmids containing P. falciparum or Toxoplasma gondii dihydrofolate reductase-thymidylate synthase (dhfr-ts) coding sequences that confer resistance to pyrimethamine. Under pyrimethamine pressure, transformed parasites were obtained that maintained the transfected plasmids as unrearranged episomes for several weeks. These parasite populations were replaced after 2 to 3 months by parasites that had incorporated the transfected DNA into nuclear chromosomes. Depending upon the particular construct used for transformation, homologous integration was detected in the P. falciparum dhfr-ts locus (chromosome 4) or in hrp3 and hrp2 sequences that were used in the plasmid constructs as gene control regions (chromosomes 13 and 8, respectively). Transformation by homologous integration sets the stage for targeted gene alterations and knock-outs that will advance understanding of P. falciparum.
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Citations
Cited by
Fidock, D. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)00084-8 · Cited by 1,456
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Gregson, A., & Plowe, C. V. (2005) · Pharmacological Reviews · doi:10.1124/pr.57.1.4 · Cited by 499
Apical membrane antigen 1 plays a central role in erythrocyte invasion by Plasmodium species
Triglia, T., Healer, J., Caruana, S. R., Hodder, A. N., Anders, R. F., Crabb, B. S., & Cowman, A. F. (2000) · Molecular Microbiology · doi:10.1046/j.1365-2958.2000.02175.x · Cited by 304
Efficient Gene Replacements in Toxoplasma gondii Strains Deficient for Nonhomologous End Joining
Fox, B. A., Ristuccia, J. G., Gigley, J. P., & Bzik, D. J. (2009) · Eukaryotic Cell · doi:10.1128/ec.00357-08 · Cited by 263
Cellular adhesive phenomena in apicomplexan parasites of red blood cells
Cooke, B. M., Mohandas, N., Cowman, A. F., & Coppel, R. L. (2005) · Veterinary Parasitology · doi:10.1016/j.vetpar.2005.07.002 · Cited by 51
Metadata
- Authors
- Wu, Y., Kirkman, L. A., & Wellems, T. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-02-06
- Volume
- 93
- Issue
- 3
- Pages
- 1130-1134
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1996079166
- Cited by
- 378
- References
- 0
- FWCI
- 35.70
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. https://app.cochat.ai/papers/10.1073/pnas.93.3.1130
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- Wu, Y., Kirkman, L. A., & Wellems, T. E. (1996). Transformation of Plasmodium falciparum malaria parasites by homologous integration of plasmids that confer resistance to pyrimethamine. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.3.1130
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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