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Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions
Open accessAbstract
A higher plant homologue to the high-affinity phosphate transporter gene of yeast ( Saccharomyces cerevisiae ) PHO84 was isolated from Arabidopsis thaliana . Expression of the Arabidopsis gene PHT1 at high levels in tobacco-cultured cells increased the rate of phosphate uptake. The uptake activity attributable to the transgene was inhibited by protonophores, suggesting an H + cotransport mechanism of phosphate uptake, and had a K m of 3.1 μM which is within limits characteristic of high-affinity transport mechanisms. These results indicate that PHT1 encodes a high-affinity phosphate transporter. The transgenic cells exhibited increased biomass production when the supply of phosphate was limited, establishing gene engineering of phosphate transport as one approach toward enhancing plant cell growth.
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Cited by
Phosphorus Uptake by Plants: From Soil to Cell
Schachtman, D. P., Reid, R. J., & Ayling, S. M. (1998) · Plant Physiology · doi:10.1104/pp.116.2.447 · Cited by 2,027
Raghothama, K. G., & Karthikeyan, A. S. (2005) · Plant and Soil · doi:10.1007/s11104-004-2005-6 · Cited by 1,640
Vance, C. P. (2001) · Plant Physiology · doi:10.1104/pp.010331 · Cited by 899
Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants
Richardson, A. E. (2001) · Australian Journal of Plant Physiology · doi:10.1071/pp01093 · Cited by 831
Functional biology of plant phosphate uptake at root and mycorrhiza interfaces
Bucher, M. (2007) · New Phytologist · doi:10.1111/j.1469-8137.2006.01935.x · Cited by 718
Metadata
- Authors
- Mitsukawa, N., Okumura, S., Shirano, Y., Sato, S., Kato, T., Harashima, S., & Shibata, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-06-24
- Volume
- 94
- Issue
- 13
- Pages
- 7098-7102
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2033004961
- Cited by
- 225
- References
- 0
- FWCI
- 13.67
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions. https://app.cochat.ai/papers/10.1073/pnas.94.13.7098
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- Mitsukawa, N., Okumura, S., Shirano, Y., Sato, S., Kato, T., Harashima, S., & Shibata, D. (1997). Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.13.7098
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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