The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Targeted overexpression of protein kinase C β2 isoform in myocardium causes cardiomyopathy
Open accessAbstract
Increased cardiovascular mortality occurs in diabetic patients with or without coronary artery disease and is attributed to the presence of diabetic cardiomyopathy. One potential mechanism is hyperglycemia that has been reported to activate protein kinase C (PKC), preferentially the β isoform, which has been associated with the development of micro- and macrovascular pathologies in diabetes mellitus. To establish that the activation of the PKCβ isoform can cause cardiac dysfunctions, we have established lines of transgenic mice with the specific overexpression of PKCβ2 isoform in the myocardium. These mice overexpressed the PKCβ2 isoform transgene by 2- to 10-fold as measured by mRNA, and proteins exhibited left ventricular hypertrophy, cardiac myocyte necrosis, multifocal fibrosis, and decreased left ventricular performance without vascular lesions. The severity of the phenotypes exhibited gene dose-dependence. Up-regulation of mRNAs for fetal type myosin heavy chain, atrial natriuretic factor, c- fos , transforming growth factor, and collagens was also observed. Moreover, treatment with a PKCβ-specific inhibitor resulted in functional and histological improvement. These findings have firmly established that the activation of the PKCβ2 isoform can cause specific cardiac cellular and functional changes leading to cardiomyopathy of diabetic or nondiabetic etiology.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction
Liang, P., & Pardee, A. B. (1992) · Science · doi:10.1126/science.1354393 · Cited by 4,984
Intracellular Signaling by Hydrolysis of Phospholipids and Activation of Protein Kinase C
Nishizuka, Y. (1992) · Science · doi:10.1126/science.1411571 · Cited by 4,472
The Transforming Growth Factor-beta Family
Massague, J. (1990) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.06.110190.003121 · Cited by 3,015
Amelioration of Vascular Dysfunctions in Diabetic Rats by an Oral PKC β Inhibitor
Ishii, H., Jirousek, M. R., Koya, D., Takagi, C., Xia, P., Clermont, A., Bursell, S.-E., Kern, T. S., Ballas, L. M., Heath, W. F., et al. (1996) · Science · doi:10.1126/science.272.5262.728 · Cited by 1,103
Self-citationProtooncogene induction and reprogramming of cardiac gene expression produced by pressure overload.
Izumo, S., Nadal-Ginard, B., & Mahdavi, V (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.2.339 · Cited by 867
Chien, K. R., Knowlton, K. U., Zhu, G., & Chien, S. (1991) · The FASEB Journal · doi:10.1096/fasebj.5.15.1835945 · Cited by 794
Inoguchi, T., Battan, R., Handler, E., Sportsman, J. R., Heath, W., & King, G. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.22.11059 · Cited by 740
Self-citationEvidence for Cardiomyopathy in Familial Diabetes Mellitus
Regan, T. J., Lyons, M. M., Ahmed, S. S., Levinson, G. E., Oldewurtel, H. A., Ahmad, M. R., & Haider, B. (1977) · Journal of Clinical Investigation · doi:10.1172/jci108843 · Cited by 728
Tissue-specific regulation of the alpha-myosin heavy chain gene promoter in transgenic mice.
Subramaniam, A., Jones, W. K., Gulick, J., Wert, S., Neumann, J., & Robbins, J. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54273-3 · Cited by 469
Cited by
Free radicals and antioxidants in normal physiological functions and human disease
Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T. D., Mazur, M., & Telser, J. (2007) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2006.07.001 · Cited by 14,269
Mechanisms of Diabetic Complications
Forbes, J. M., & Cooper, M. E. (2013) · Physiological Reviews · doi:10.1152/physrev.00045.2011 · Cited by 2,890
Bernardo, B. C., Weeks, K. L., Pretorius, L., & McMullen, J. R. (2010) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2010.04.005 · Cited by 894
Du, X., Matsumura, T., Edelstein, D., Rossetti, L., Zsengellér, Z., Szabó, C., & Brownlee, M. (2003) · Journal of Clinical Investigation · doi:10.1172/jci18127 · Cited by 832
The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice
Bueno, O. F. (2000) · The EMBO Journal · doi:10.1093/emboj/19.23.6341 · Cited by 774
Metadata
- Authors
- Wakasaki, H., Koya, D., Schoen, F. J., Jirousek, M. R., Ways, D. K., Hoit, B. D., Walsh, R. A., & King, G. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-08-19
- Volume
- 94
- Issue
- 17
- Pages
- 9320-9325
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2049401019
- Cited by
- 379
- References
- 25
- FWCI
- 15.11
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Targeted overexpression of protein kinase C β2 isoform in myocardium causes cardiomyopathy. https://app.cochat.ai/papers/10.1073/pnas.94.17.9320
- The paper
- Wakasaki, H., Koya, D., Schoen, F. J., Jirousek, M. R., Ways, D. K., Hoit, B. D., Walsh, R. A., & King, G. L. (1997). Targeted overexpression of protein kinase C β2 isoform in myocardium causes cardiomyopathy. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.17.9320
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
