The DOI matches its Crossref record.
Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome
Open accessAbstract
A strategy for cloning and mutagenesis of an infectious herpesvirus genome is described. The mouse cytomegalovirus genome was cloned and maintained as a 230 kb bacterial artificial chromosome (BAC) in E. coli . Transfection of the BAC plasmid into eukaryotic cells led to a productive virus infection. The feasibility to introduce targeted mutations into the BAC cloned virus genome was shown by mutation of the immediate-early 1 gene and generation of a mutant virus. Thus, the complete construction of a mutant herpesvirus genome can now be carried out in a controlled manner prior to the reconstitution of infectious progeny. The described approach should be generally applicable to the mutagenesis of genomes of other large DNA viruses.
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Cited by
A point mutation in a herpesvirus polymerase determines neuropathogenicity.
Goodman, L. B., Loregian, A., Perkins, G. A., Nugent, J., Buckles, E. L., Mercorelli, B., Kydd, J. H., Palù, G., Smith, K. C., Osterrieder, N., et al. (2007) · PLoS Pathogens · doi:10.1371/journal.ppat.0030160 · Cited by 223
Simon, C. O., Holtappels, R., Tervo, H.-M., Böhm, V., Däubner, T., Oehrlein-Karpi, S. A., Kühnapfel, B., Renzaho, A., Strand, D., Podlech, J., et al. (2006) · Journal of Virology · doi:10.1128/jvi.01248-06 · Cited by 182
Metadata
- Authors
- Messerle, M., Crnkovic, I., Hammerschmidt, W., Ziegler, H., & Koszinowski, U. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-12-23
- Volume
- 94
- Issue
- 26
- Pages
- 14759-14763
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2057208302
- Cited by
- 426
- References
- 0
- FWCI
- 12.59
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome. https://app.cochat.ai/papers/10.1073/pnas.94.26.14759
- The paper
- Messerle, M., Crnkovic, I., Hammerschmidt, W., Ziegler, H., & Koszinowski, U. H. (1997). Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.26.14759
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
