The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Persistence in muscle of an adenoviral vector that lacks all viral genes
Open accessAbstract
Genetic correction of inherited muscle diseases, such as Duchenne muscular dystrophy, will require long term expression of the recombinant protein following gene transfer. We have shown previously that a new adenoviral vector that lacks all viral genes expressed both full-length dystrophin and β-galactosidase in mdx (dystrophin-deficient) mouse muscle. We observed a significant histologic improvement of vector-transduced mdx muscle before the eventual loss of vector-encoded transgene expression. In this study, we investigated whether an immunological response against vector-encoded β-galactosidase contributed to the loss of vector expression and affected vector persistence in muscle. Intramuscular vector injection in control normal mice resulted in an early and complete loss of β-galactosidase expression accompanied by predominantly CD4 + and CD8 + lymphocytic infiltration and a significant loss of vector DNA. In contrast, intramuscular vector injection in lacZ transgenic mice resulted in persistent expression of β-galactosidase for at least 84 days with no evidence of inflammation or significant loss of vector DNA. Our studies demonstrate that, in the absence of an immune response induced by β-galactosidase expression, an adenoviral vector lacking all viral genes is stably maintained in muscle.
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Cites
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Self-citationEfficient adenovirus-mediated transfer of a human minidystrophin gene to skeletal muscle of mdx mice
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Cited by
Gene therapy - promises, problems and prospects
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Self-citationImmunity to adenovirus and adeno-associated viral vectors: implications for gene therapy
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Metadata
- Authors
- Chen, H.-H., Mack, L. M., Kelly, R., Ontell, M., Kochanek, S., & Clemens, P. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-03-04
- Volume
- 94
- Issue
- 5
- Pages
- 1645-1650
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2035600956
- Cited by
- 291
- References
- 26
- FWCI
- 19.71
- Type
- journal article
- Language
- en
- Open access
- green
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- Chen, H.-H., Mack, L. M., Kelly, R., Ontell, M., Kochanek, S., & Clemens, P. R. (1997). Persistence in muscle of an adenoviral vector that lacks all viral genes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.5.1645
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