A Xenopus oocyte β subunit: Evidence for a role in the assembly/expression of voltage-gated calcium channels that is separate from its role as a regulatory subunit

Abstract

Two closely related β subunit mRNAs (xo28 and xo32) were identified in Xenopus oocytes by molecular cloning. One or both appear to be expressed as active proteins, because: ( i ) injection of Xenopus β antisense oligonucleotides, but not of sense or unrelated oligonucleotides, significantly reduced endogenous oocyte voltage-gated Ca 2+ channel (VGCC) currents and obliterated VGCC currents that arise after injection of mammalian α 1 cRNAs (α 1C and α 1E ); ( ii ) coinjection of a Xenopus β antisense oligonucleotide and excess rat β cRNA rescued expression of α 1 Ca 2+ channel currents; and ( iii ) coinjection of mammalian α 1 cRNA with cRNA encoding either of the two Xenopus β subunits facilitated both activation and inactivation of Ca 2+ channel currents by voltage, as happens with most mammalian β subunits. The Xenopus β subunit cDNAs (β3xo cDNAs) predict proteins of 484 aa that differ in only 22 aa and resemble most closely the sequence of the mammalian type 3 β subunit. We propose that “α 1 alone” channels are in fact tightly associated α 1 β3xo channels, and that effects of exogenous β subunits are due to formation of higher-order [α 1 β]β n complexes with an unknown contribution of β3xo. It is thus possible that functional mammalian VGCCs, rather than having subunit composition α 1 β, are [α 1 β]β n complexes that associate with α 2 δ and, as appropriate, other tissue-specific accessory proteins. In support of this hypothesis, we discovered that the last 277-aa of α 1E have a β subunit binding domain. This β binding domain is distinct from the previously known interaction domain located between repeats I and II of calcium channel α 1 subunits.