The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Expression of a borage desaturase cDNA containing an N-terminal cytochrome b 5 domain results in the accumulation of high levels of Δ 6 -desaturated fatty acids in transgenic tobacco
Open accessAbstract
γ-Linolenic acid (GLA; C18:3 Δ 6,9,12 ) is a component of the seed oils of evening primrose ( Oenothera spp.), borage ( Borago officinalis L.), and some other plants. It is widely used as a dietary supplement and for treatment of various medical conditions. GLA is synthesized by a Δ 6 -fatty acid desaturase using linoleic acid (C18:2 Δ 9,12 ) as a substrate. To enable the production of GLA in conventional oilseeds, we have isolated a cDNA encoding the Δ 6 -fatty acid desaturase from developing seeds of borage and confirmed its function by expression in transgenic tobacco plants. Analysis of leaf lipids from a transformed plant demonstrated the accumulation of GLA and octadecatetraenoic acid (C18:4 Δ 6,9,12,15 ) to levels of 13.2% and 9.6% of the total fatty acids, respectively. The borage Δ 6 -fatty acid desaturase differs from other desaturase enzymes, characterized from higher plants previously, by the presence of an N-terminal domain related to cytochrome b 5 .
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Citations
Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Gammar linolenic acid—occurrence and physical and chemical properties
Gunstone, F. D. (1992) · Progress in Lipid Research · doi:10.1016/0163-7827(92)90007-6 · Cited by 159
Cited by
DESATURATION AND RELATED MODIFICATIONS OF FATTY ACIDS
Shanklin, J., & Cahoon, E. B. (1998) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.49.1.611 · Cited by 916
Structure and expression of fatty acid desaturases
Los, D. A., & Murata, N. (1998) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/s0005-2760(98)00091-5 · Cited by 558
New roles for cis-jasmone as an insect semiochemical and in plant defense
Birkett, M. A., Campbell, C. A. M., Chamberlain, K., Guerrieri, E., Hick, A. J., Martin, J. L., Matthes, M., Napier, J. A., Pettersson, J., Pickett, J. A., et al. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.160241697 · Cited by 451
Self-citationProduction of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants
Qi, B., Fraser, T., Mugford, S., Dobson, G., Sayanova, O., Butler, J., Napier, J. A., Stobart, A. K., & Lazarus, C. M. (2004) · Nature Biotechnology · doi:10.1038/nbt972 · Cited by 426
Self-citationBergé, J.-P., & Barnathan, G. (2005) · Advances in Biochemical Engineering/Biotechnology · doi:10.1007/b135782 · Cited by 401
Metadata
- Authors
- Sayanova, O., Smith, M. A., Lapinskas, P., Stobart, A. K., Dobson, G., Christie, W. W., Shewry, P. R., & Napier, J. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-04-15
- Volume
- 94
- Issue
- 8
- Pages
- 4211-4216
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2046012971
- Cited by
- 276
- References
- 2
- FWCI
- 8.94
- Type
- journal article
- Language
- en
- Open access
- green
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- Sayanova, O., Smith, M. A., Lapinskas, P., Stobart, A. K., Dobson, G., Christie, W. W., Shewry, P. R., & Napier, J. A. (1997). Expression of a borage desaturase cDNA containing an N-terminal cytochrome b 5 domain results in the accumulation of high levels of Δ 6 -desaturated fatty acids in transgenic tobacco. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.8.4211
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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