The DOI matches its Crossref record.
Mutation of the polo-box disrupts localization and mitotic functions of the mammalian polo kinase Plk
Open accessAbstract
Members of the polo subfamily of protein kinases play pivotal roles in cell proliferation. In addition to the kinase domain, polo kinases have a strikingly conserved sequence in the noncatalytic domain, termed the polo-box. The function of the polo-box is currently undefined. The mammalian polo-like kinase Plk is a functional homologue of Saccharomyces cerevisiae Cdc5. Here, we show that Plk localizes at the spindle poles and cytokinetic neck filaments. Without impairing kinase activity, a conservative mutation in the polo-box disrupts the capacity of Plk to complement the defect associated with a cdc5–1 temperature-sensitive mutation and to localize to these subcellular structures. Our data provide evidence that the polo-box plays a critical role in Plk function, likely by directing its subcellular localization.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Polo on the Rise—from Mitotic Entry to Cytokinesis with Plk1
Petronczki, M., Lénárt, P., & Peters, J.-M. (2008) · Developmental Cell · doi:10.1016/j.devcel.2008.04.014 · Cited by 485
Closing Mitosis: The Functions of the Cdc14 Phosphatase and Its Regulation
Stegmeier, F., & Amon, A. (2004) · Annual Review of Genetics · doi:10.1146/annurev.genet.38.072902.093051 · Cited by 432
Polo-like kinases (Plks) and cancer
Takai, N., Hamanaka, R., Yoshimatsu, J., & Miyakawa, I. (2005) · Oncogene · doi:10.1038/sj.onc.1208272 · Cited by 386
Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8
Helmke, C., Raab, M., Rödel, F., Matthess, Y., Oellerich, T., Mandal, R., Sanhaji, M., Urlaub, H., Rödel, C., Becker, S., et al. (2016) · Cell Research · doi:10.1038/cr.2016.78 · Cited by 44
Metadata
- Authors
- Lee, K. S., Grenfell, T. Z., Yarm, F. R., & Erikson, R. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-08-04
- Volume
- 95
- Issue
- 16
- Pages
- 9301-9306
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2069449346
- Cited by
- 245
- References
- 0
- FWCI
- 3.39
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Mutation of the polo-box disrupts localization and mitotic functions of the mammalian polo kinase Plk. https://app.cochat.ai/papers/10.1073/pnas.95.16.9301
- The paper
- Lee, K. S., Grenfell, T. Z., Yarm, F. R., & Erikson, R. L. (1998). Mutation of the polo-box disrupts localization and mitotic functions of the mammalian polo kinase Plk. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.16.9301
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
